Phytuberol
Phytuberol is an antibacterial agent and unsaturated sesquiterpene alcohol. As a phytoalexin, phytuberol participates in plant defense responses by inhibiting the growth of pathogenic fungi (e.g., Phytophthora infestans, Helminthosporium spp.). Its accumulation increases significantly in potato tuber slices treated with Ethephon (HY-W131845) and fungal elicitors, and correlates with tissue browning and disease resistance responses. Hytuberol can be used in studies related to bacterial wilt, tobacco wildfire disease, potato late blight, and potato-fungus interactions.
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- CAS No.: 56857-64-8
- Formule: C15H24O3
- Masse moléculaire:252.35
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
In Vitro
Phytuberol (2 mL l-1 10% (w/v) Rhizoctonia bataticola fungal elicitor + 100 mg l-1 β-cyclodextrin; 48 h) production is induced in Solanum tuberosum hairy root cultures, with peak production of ~40 μg g-1 dry wt achieved under these conditions[1].
Phytuberol (2 days after inoculation) accumulation is induced by inoculation with Pseudomonas solanacearum U-7 in Burley 21 and Mito 3 tobacco callus (3.83 μg/g dry wt and 1.15 μg/g dry wt, respectively), but not in Bright Yellow 4 or Coker 319 tobacco callus[2].
Phytuberol (4 days after inoculation; 3-6 weeks post-transplant growth period) accumulation induced by Pseudomonas solanacearum U-7 in Burley 21 tobacco callus is stable at ~1.6-1.8 μg/g dry wt in 3-5-week-old tissues, but drops to a trace level in 6-week-old tissues[2].
Phytuberol (0 to 4 days after inoculation) accumulation in Burley 21 tobacco callus inoculated with Pseudomonas solanacearum U-7 increases over time, reaching its maximum 4 days after inoculation[2].
Phytuberol (4 days after inoculation) accumulation in Burley 21 tobacco callus is induced to high levels (6.7-6.8 μg/g dry wt) by inoculation with Pseudomonas solanacearum strains (M23R and U-7), while inoculation with Pseudomonas syringae pv. tabaci strains (6602 and 7246) induces low phytuberol accumulation (0.24-0.41 μg/g dry wt)[2].
Phytuberol (36-84 h) accumulates to increasing levels in Ethrel-treated Kennebec potato tuber slices inoculated with incompatible race 4 of Phytophthora infestans, reaching 37 μg/g fresh weight after 84 hours of incubation[3].
Phytuberol (72 h) accumulates to 43 μg/g fresh weight in Ethrel-treated Kennebec potato tuber slices and 95 μg/g fresh weight in Ethrel-treated Russet Burbank potato tuber slices inoculated with Helminthosporium victoriae after 72 hours of incubation[3].
Phytuberol (72 h) accumulates to 41 μg/g fresh weight in Ethrel-treated Kennebec potato tuber slices and 67 μg/g fresh weight in Ethrel-treated Russet Burbank potato tuber slices inoculated with Helminthosporium carbonum after 72 hours of incubation[3].
Phytuberol (36-84 h) accumulates to increasing levels in Ethrel-treated Kennebec and Russet Burbank potato tuber slices treated with autoclaved cell-free sonicates of Phytophthora infestans race 4, reaching 17 μg/g and 14 μg/g fresh weight respectively after 84 hours of incubation[3].
Phytuberol (72 h) accumulates to 25 μg/g fresh weight in Ethrel-treated Kennebec potato tuber slices treated with autoclaved cell-free sonicates of Pythium aphanidermatum after 72 hours of incubation[3].
Phytuberol (72 h) accumulates to 23 μg/g fresh weight in Ethrel-treated Kennebec potato tuber slices treated with autoclaved cell-free sonicates of Achlya flagellata after 72 hours of incubation[3].
Phytuberol accumulates in Ethrel-treated Kennebec potato tuber slices treated with autoclaved cell-free sonicates of Phytophthora infestans race 4 at 14°C, 19°C, and 25°C, with maximum accumulation (31 μg/g fresh weight) occurring at 19°C; no phytuberol is detected at 30°C or 36°C[3].
Phytuberol (100 μliters/liter; 72 h) accumulates to 52 μg/g fresh weight in Kennebec potato tuber slices treated with autoclaved cell-free sonicates of Phytophthora infestans race 4 and exposed to 100 μliters/liter ethylene after 72 hours of incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 56857-64-8
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Masse moléculaire 252.35
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Formule C15H24O3
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SMILES
C[C@@]12[C@]3(C[C@@H](CC2)C(C)(O)C)[C@](OC1)(C=CO3)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)