Chrolactomycin
Chrolactomycin is found in the strain of Streptomyces sp. 569N-3. The IC50 of Chrolactomycin for ACHN, A431, McF-7 and T24 cells are 1.2 μM, 1.6 μM, 0.69 μM and 0.45 μM, respectively. Chrolactomycin has only anti-Gram-positive bacteria activity with the MIC of 5.2-10.4 μg/mL.
For research use only. We do not sell to patients.
- CAS No.: 227606-82-8
- Formula: C24H32O7
- Molecular Weight:432.51
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
0.5 μM
Compound: 10
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Inhibition of human telomerase isolated from human A431 cellular extracts after 30 mins by TRAP assay
Inhibition of human telomerase isolated from human A431 cellular extracts after 30 mins by TRAP assay
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[PMID: 24053596] |
| A-431 | IC50 |
0.5 μM
Compound: Chrolactomycin
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Inhibition of telomerase extracted from human A431 cells after 30 mins by TRAP assay
Inhibition of telomerase extracted from human A431 cells after 30 mins by TRAP assay
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10.1039/C0MD00241K |
Chemical Information
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CAS No. 227606-82-8
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Molecular Weight 432.51
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Formula C24H32O7
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SMILES
CO[C@@]12[C@]3([H])[C@@]4(OC2=O)[C@](CCC[C@H](C[C@H]([C@@](C(C1=O)=C)([H])O3)C)C)([H])C=C([C@H](C4)C)C(O)=O
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Structure Classification
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Initial Source
Streptomyces
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Chrolactomycin Related Classifications
HY-N14056 Related Classifications
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