1. Cytokines
  2. Recombinant Human Basic Fibroblast Growth Factor

Recombinant Human Basic Fibroblast Growth Factor

Cat. No.: HY-P7004
Handling Instructions

Recombinant human Basic Fibroblast Growth Factor is a potent mitogen and chemoattractant for endothelial cells as well as fibroblasts.

For research use only. We do not sell to patients.

Cytokines-MedChemExpress
Size Price Stock Quantity
10 μg $90 In-stock
Estimated Time of Arrival: December 31
50 μg $190 In-stock
Estimated Time of Arrival: December 31
100 μg $290 In-stock
Estimated Time of Arrival: December 31
250 μg   Get quote  

* Please select Quantity before adding items.

  • Technical Parameters

  • Properties

  • Description

Synonyms
rHubFGF; HBGF-2; FGF-2; FGF-b; FGF-basic
Species

Human

Source

E. coli

Accession

P09038

Gene ID

2247

Molecular Weight

Approximately 16.5 kDa

AA Sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Biological Activity
The ED50 is <0.25 ng/mL as measured by 3T3 cells, corresponding to a specific activity of > 4.0 × 106 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconsititution

Reconstitute the lyophilized recombinant Human Basic Fibroblast Growth Factor (rHubFGF) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA.

Storage & Stability

Lyophilized recombinant Human Basic Fibroblast Growth Factor (rHubFGF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Recombinant Human Basic Fibroblast Growth Factor is a potent mitogen and chemoattractant for endothelial cells as well as fibroblasts. Basic fibroblast growth factor (bFGF) is a heparin-binding mitogen for mesoderm-derived cells, also acts as a mitogen, differentiation inducing and maintenance factor for many neuroectodermal cells including glial cells, neurons, paraneurons, and their tumor counterparts. The molecule is expressed in several types of neuroectodermal cells. bFGF occurs in many neuronal target tissues, and can prevent ontogenetic as well as lesion-induced neuron death[1]. In vitro, basic fibroblast growth factor induces an angiogenic phenotype in endothelial cells, which includes chemotaxis, mitogenesis, protease production, beta-integrin expression, and tube formation in three-dimensional gels. It acts by binding to specific tyrosine kinase receptors and to cell-associated heparan sulfate proteoglycans[2].

Documentation
References
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Product Name:
Recombinant Human Basic Fibroblast Growth Factor
Cat. No.:
HY-P7004
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