DBCO-NHCO-PEG5-NHS ester
Based on 1 Customer Validation
DBCO-NHCO-PEG5-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-NHCO-PEG5-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- CAS No.: 1378531-80-6
- Formula: C36H43N3O11
- Molecular Weight:693.74
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-140272
DBCO-PEG4-NHS esterPROTAC Linker -
HY-42973
DBCO-NHS esterADC Linker -
HY-115524
DBCO-NHS ester 2ADC linker -
HY-123687
DBCO-Sulfo-NHS ester sodiumADC Linker -
HY-116270
DBCO-MaleimideADC Linker -
HY-151835
DBCO-PEG24-NHS esterClick Chemical Agent -
HY-126885
DBCO-PEG5-NHS esterADC/PROTAC Linker -
HY-170528
DBCO-PEG3-NHSPROTAC Linker -
HY-151827
DBCO-PEG2-NHS esterAzide Compound -
HY-130396
DBCO-PEG4-C2-acidPROTAC Linker -
HY-140271
DBCO-PEG1-NHS esterPROTAC Linkers -
HY-140279
DBCO-PEG4-alcoholPROTAC Linker
All PROTAC Linkers Isoforms
More
Biological Activity
Description
IC50 & Target
|
PEGs |
In Vitro
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 1378531-80-6
-
Molecular Weight 693.74
-
Formula C36H43N3O11
-
SMILES
O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCOCCC(NCCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DBCO-NHCO-PEG5-NHS ester Related Classifications
HY-140275 Related Classifications
Powered by Bioz