DMT-2′Fluoro-dU Phosphoramidite
Based on 1 Customer Validation
DMT-2’ Fluoro-dU Phosphoramidite (2'-F-dU Phosphoramidite) is a uridine phosphoramidite with a fluorine in the 2’ position. DMT-2’ Fluoro-dU Phosphoramidite could be used for nucleoside modification and synthesis of DNA.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 146954-75-8
- Formula: C39H46FN4O8P
- Molecular Weight:748.78
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Fluorination is one of the most common chemical derivatizations that have been widely used in pharmaceutical chemistry, due to the unique biophysical and biochemical properties of fluorine element, like small size and extreme electronegativity. The ongoing development of novel fluorinated nucleic acid molecules is significant, in order to benefit the fields of innovative drug discovery and basic biochemical studies[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 146954-75-8
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Appearance Solid
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Molecular Weight 748.78
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Formula C39H46FN4O8P
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Color White to yellow
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SMILES
F[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C2=CC=C(OC)C=C2)(C3=CC=C(OC)C=C3)C4=CC=CC=C4)O[C@H]1N5C(NC(C=C5)=O)=O
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Synonyms
2'-F-dU Phosphoramidite
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (133.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.3355 mL | 6.6775 mL | 13.3551 mL | 33.3876 mL |
| 5 mM | 0.2671 mL | 1.3355 mL | 2.6710 mL | 6.6775 mL | |
| 10 mM | 0.1336 mL | 0.6678 mL | 1.3355 mL | 3.3388 mL | |
| 15 mM | 0.0890 mL | 0.4452 mL | 0.8903 mL | 2.2258 mL | |
| 20 mM | 0.0668 mL | 0.3339 mL | 0.6678 mL | 1.6694 mL | |
| 25 mM | 0.0534 mL | 0.2671 mL | 0.5342 mL | 1.3355 mL | |
| 30 mM | 0.0445 mL | 0.2226 mL | 0.4452 mL | 1.1129 mL | |
| 40 mM | 0.0334 mL | 0.1669 mL | 0.3339 mL | 0.8347 mL | |
| 50 mM | 0.0267 mL | 0.1336 mL | 0.2671 mL | 0.6678 mL | |
| 60 mM | 0.0223 mL | 0.1113 mL | 0.2226 mL | 0.5565 mL | |
| 80 mM | 0.0167 mL | 0.0835 mL | 0.1669 mL | 0.4173 mL | |
| 100 mM | 0.0134 mL | 0.0668 mL | 0.1336 mL | 0.3339 mL |
DMT-2′Fluoro-dU Phosphoramidite Related Classifications
HY-45409 Related Classifications
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