DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite
Based on 1 Customer Validation
DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite is an RNA phosphoramidite monomer. DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite enables site-specific introduction of 2'-O-C22 lipophilic modification at cytidine positions during oligonucleotide synthesis, which is used to construct double-stranded RNA (dsRNA) molecules with extrahepatic delivery capability. DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite supports target gene silencing in skeletal muscle, cardiac muscle and adipose tissue by enhancing the lipophilicity and tissue uptake efficiency of dsRNA. Construction and mechanism study of DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite nucleic acid silencing molecules.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2923115-93-7
- Formula: C63H94N5O9P
- Molecular Weight:1096.42
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite (Compound 114) is synthesized via a phosphoramidite coupling reaction with 2-cyanoethyl N,N-diisopropylchlorophosphoramidite. The reaction progress is verified through work-up and purification procedures. After quenching with saturated sodium bicarbonate, extraction with ethyl acetate, washing with saturated sodium chloride, and drying over anhydrous sodium sulfate, the crude product is purified by silica gel column chromatography to yield a white foamy solid. The product is characterized by 1H NMR (600 MHz, DMSO) and 31P NMR (243 MHz, DMSO), with 31P NMR chemical shifts measured at δ 149.23 and 147.94[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2923115-93-7
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Appearance Solid
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Molecular Weight 1096.42
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Formula C63H94N5O9P
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=C(C=C2)OC)(C3=CC=CC=C3)OC[C@@H]4[C@@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](OCCCCCCCCCCCCCCCCCCCCCC)[C@H](N5C(N=C(NC(C)=O)C=C5)=O)O4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 87.5 mg/mL (79.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9121 mL | 4.5603 mL | 9.1206 mL | 22.8015 mL |
| 5 mM | 0.1824 mL | 0.9121 mL | 1.8241 mL | 4.5603 mL | |
| 10 mM | 0.0912 mL | 0.4560 mL | 0.9121 mL | 2.2801 mL | |
| 15 mM | 0.0608 mL | 0.3040 mL | 0.6080 mL | 1.5201 mL | |
| 20 mM | 0.0456 mL | 0.2280 mL | 0.4560 mL | 1.1401 mL | |
| 25 mM | 0.0365 mL | 0.1824 mL | 0.3648 mL | 0.9121 mL | |
| 30 mM | 0.0304 mL | 0.1520 mL | 0.3040 mL | 0.7600 mL | |
| 40 mM | 0.0228 mL | 0.1140 mL | 0.2280 mL | 0.5700 mL | |
| 50 mM | 0.0182 mL | 0.0912 mL | 0.1824 mL | 0.4560 mL | |
| 60 mM | 0.0152 mL | 0.0760 mL | 0.1520 mL | 0.3800 mL |
DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite Related Classifications
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