2. Others Cell Cycle/DNA Damage
  3. Phosphoramidites DNA/RNA Synthesis
  4. DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite

DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite 

Cat. No.: HY-164582 Purity: 98.0%
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DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite is an RNA phosphoramidite monomer. DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite enables site-specific introduction of 2'-O-C22 lipophilic modification at cytidine positions during oligonucleotide synthesis, which is used to construct double-stranded RNA (dsRNA) molecules with extrahepatic delivery capability. DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite supports target gene silencing in skeletal muscle, cardiac muscle and adipose tissue by enhancing the lipophilicity and tissue uptake efficiency of dsRNA. Construction and mechanism study of DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite nucleic acid silencing molecules.

For research use only. We do not sell to patients.

DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite

DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite Chemical Structure

CAS No. : 2923115-93-7

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DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite Related Antibodies

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Description

DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite is an RNA phosphoramidite monomer. DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite enables site-specific introduction of 2'-O-C22 lipophilic modification at cytidine positions during oligonucleotide synthesis, which is used to construct double-stranded RNA (dsRNA) molecules with extrahepatic delivery capability. DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite supports target gene silencing in skeletal muscle, cardiac muscle and adipose tissue by enhancing the lipophilicity and tissue uptake efficiency of dsRNA. Construction and mechanism study of DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite nucleic acid silencing molecules[1].

In Vitro

DMTr-2'-O-C22-rC (Ac)-3'-CE-Phosphoramidite (Compound 114) is synthesized via a phosphoramidite coupling reaction with 2-cyanoethyl N,N-diisopropylchlorophosphoramidite. The reaction progress is verified through work-up and purification procedures. After quenching with saturated sodium bicarbonate, extraction with ethyl acetate, washing with saturated sodium chloride, and drying over anhydrous sodium sulfate, the crude product is purified by silica gel column chromatography to yield a white foamy solid. The product is characterized by 1H NMR (600 MHz, DMSO) and 31P NMR (243 MHz, DMSO), with 31P NMR chemical shifts measured at δ 149.23 and 147.94[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite Related Antibodies
Molecular Weight

1096.42

Formula

C63H94N5O9P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC(C=C1)=CC=C1C(C2=CC=C(C=C2)OC)(C3=CC=CC=C3)OC[C@@H]4[C@@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](OCCCCCCCCCCCCCCCCCCCCCC)[C@H](N5C(N=C(NC(C)=O)C=C5)=O)O4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 87.5 mg/mL (79.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9121 mL 4.5603 mL 9.1206 mL
5 mM 0.1824 mL 0.9121 mL 1.8241 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9121 mL 4.5603 mL 9.1206 mL 22.8015 mL
5 mM 0.1824 mL 0.9121 mL 1.8241 mL 4.5603 mL
10 mM 0.0912 mL 0.4560 mL 0.9121 mL 2.2801 mL
15 mM 0.0608 mL 0.3040 mL 0.6080 mL 1.5201 mL
20 mM 0.0456 mL 0.2280 mL 0.4560 mL 1.1401 mL
25 mM 0.0365 mL 0.1824 mL 0.3648 mL 0.9121 mL
30 mM 0.0304 mL 0.1520 mL 0.3040 mL 0.7600 mL
40 mM 0.0228 mL 0.1140 mL 0.2280 mL 0.5700 mL
50 mM 0.0182 mL 0.0912 mL 0.1824 mL 0.4560 mL
60 mM 0.0152 mL 0.0760 mL 0.1520 mL 0.3800 mL
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DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMTr-2'-O-C22-rC(Ac)-3'-CE-Phosphoramidite2923115-93-7PhosphoramiditesDNA/RNA SynthesisdsRNA agentsC22 hydrocarbon chain modificationphosphoramidite building blockInhibitorinhibitorinhibit

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