DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite

Cat. No.: HY-164583 Pureza: 98.0%: Ficha de datos
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DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite is an RNA phosphoramidite monomer. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite allows site-specific introduction of a 2'-O-C22 lipophilic modification at adenosine positions to construct double-stranded RNA (dsRNA) molecules with extrahepatic delivery capability. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite supports target gene silencing in skeletal muscle, cardiac muscle and adipose tissue by enhancing the lipophilicity and tissue uptake efficiency of dsRNA. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite can be used for the construction and mechanism research of nucleic acid silencing molecules.

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DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite Estructura química

No. CAS : 2923115-67-5

Tamaño	Stock	Cantidad
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Descripciòn

In Vitro

DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite (Compound 103) is synthesized via a phosphoramidite coupling reaction with 2-cyanoethyl N,N-diisopropylchlorophosphoramidite. TLC monitoring shows that the starting material Compound 102 is completely consumed, and a new major spot (Rf = 0.4) appears in the 40% ethyl acetate/n-hexane system. The reaction is quenched with saturated sodium bicarbonate, washed with water and brine, dried over sodium sulfate, and concentrated under reduced pressure. The crude product is purified by silica gel column chromatography to yield the product as a white solid. The product is characterized by ¹H NMR (600 MHz, CD₃CN), ³¹P NMR (243 MHz, CD₃CN) and LC-MS (ESI), with a measured [M+H]⁺ = 1182.6^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

1182.52

Fòrmula

C₆₉H₉₆N₇O₈P

No. CAS

2923115-67-5

Appearance

Solid

Color

White to off-white

SMILES

COC(C=C1)=CC=C1C(C2=CC=C(C=C2)OC)(C3=CC=CC=C3)OC[C@@H]4[C@@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](OCCCCCCCCCCCCCCCCCCCCCC)[C@H](N5C6=NC=NC(NC(C7=CC=CC=C7)=O)=C6N=C5)O4

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (84.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8457 mL	4.2283 mL	8.4565 mL
5 mM	0.1691 mL	0.8457 mL	1.6913 mL
10 mM	0.0846 mL	0.4228 mL	0.8457 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (4.23 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 98.0%

Select Batch:

Ficha de datos (272 KB) SDS (252 KB)

COA (249 KB) HNMR (327 KB) CNMR (334 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Jayaprakash K. NAIR, et al. Extra-hepatic delivery iRNA compositions and methods of use thereof. WO2023064530A1. WIPO (PCT). 2023-04-20.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.8457 mL	4.2283 mL	8.4565 mL	21.1413 mL
	5 mM	0.1691 mL	0.8457 mL	1.6913 mL	4.2283 mL
	10 mM	0.0846 mL	0.4228 mL	0.8457 mL	2.1141 mL
	15 mM	0.0564 mL	0.2819 mL	0.5638 mL	1.4094 mL
	20 mM	0.0423 mL	0.2114 mL	0.4228 mL	1.0571 mL
	25 mM	0.0338 mL	0.1691 mL	0.3383 mL	0.8457 mL
	30 mM	0.0282 mL	0.1409 mL	0.2819 mL	0.7047 mL
	40 mM	0.0211 mL	0.1057 mL	0.2114 mL	0.5285 mL
	50 mM	0.0169 mL	0.0846 mL	0.1691 mL	0.4228 mL
	60 mM	0.0141 mL	0.0705 mL	0.1409 mL	0.3524 mL
	80 mM	0.0106 mL	0.0529 mL	0.1057 mL	0.2643 mL