DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite
Based on 1 Customer Validation
DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite is an RNA phosphoramidite monomer. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite allows site-specific introduction of a 2'-O-C22 lipophilic modification at adenosine positions to construct double-stranded RNA (dsRNA) molecules with extrahepatic delivery capability. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite supports target gene silencing in skeletal muscle, cardiac muscle and adipose tissue by enhancing the lipophilicity and tissue uptake efficiency of dsRNA. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite can be used for the construction and mechanism research of nucleic acid silencing molecules.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2923115-67-5
- Formula: C69H96N7O8P
- Molecular Weight:1182.52
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite (Compound 103) is synthesized via a phosphoramidite coupling reaction with 2-cyanoethyl N,N-diisopropylchlorophosphoramidite. TLC monitoring shows that the starting material Compound 102 is completely consumed, and a new major spot (Rf = 0.4) appears in the 40% ethyl acetate/n-hexane system. The reaction is quenched with saturated sodium bicarbonate, washed with water and brine, dried over sodium sulfate, and concentrated under reduced pressure. The crude product is purified by silica gel column chromatography to yield the product as a white solid. The product is characterized by 1H NMR (600 MHz, CD3CN), 31P NMR (243 MHz, CD3CN) and LC-MS (ESI), with a measured [M+H]+ = 1182.6[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2923115-67-5
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Appearance Solid
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Molecular Weight 1182.52
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Formula C69H96N7O8P
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=C(C=C2)OC)(C3=CC=CC=C3)OC[C@@H]4[C@@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](OCCCCCCCCCCCCCCCCCCCCCC)[C@H](N5C6=NC=NC(NC(C7=CC=CC=C7)=O)=C6N=C5)O4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (84.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8457 mL | 4.2283 mL | 8.4565 mL | 21.1413 mL |
| 5 mM | 0.1691 mL | 0.8457 mL | 1.6913 mL | 4.2283 mL | |
| 10 mM | 0.0846 mL | 0.4228 mL | 0.8457 mL | 2.1141 mL | |
| 15 mM | 0.0564 mL | 0.2819 mL | 0.5638 mL | 1.4094 mL | |
| 20 mM | 0.0423 mL | 0.2114 mL | 0.4228 mL | 1.0571 mL | |
| 25 mM | 0.0338 mL | 0.1691 mL | 0.3383 mL | 0.8457 mL | |
| 30 mM | 0.0282 mL | 0.1409 mL | 0.2819 mL | 0.7047 mL | |
| 40 mM | 0.0211 mL | 0.1057 mL | 0.2114 mL | 0.5285 mL | |
| 50 mM | 0.0169 mL | 0.0846 mL | 0.1691 mL | 0.4228 mL | |
| 60 mM | 0.0141 mL | 0.0705 mL | 0.1409 mL | 0.3524 mL | |
| 80 mM | 0.0106 mL | 0.0529 mL | 0.1057 mL | 0.2643 mL |
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