DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite
Based on 1 Customer Validation
DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite is an RNA phosphoramidite monomer. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite allows site-specific introduction of a 2'-O-C22 lipophilic modification at adenosine positions to construct double-stranded RNA (dsRNA) molecules with extrahepatic delivery capability. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite supports target gene silencing in skeletal muscle, cardiac muscle and adipose tissue by enhancing the lipophilicity and tissue uptake efficiency of dsRNA. DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite can be used for the construction and mechanism research of nucleic acid silencing molecules.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.0%
- CAS 番号: 2923115-67-5
- 分子式: C69H96N7O8P
- 分子量:1182.52
-
保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示
More
生物活性
製品説明
体外実験
DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite (Compound 103) is synthesized via a phosphoramidite coupling reaction with 2-cyanoethyl N,N-diisopropylchlorophosphoramidite. TLC monitoring shows that the starting material Compound 102 is completely consumed, and a new major spot (Rf = 0.4) appears in the 40% ethyl acetate/n-hexane system. The reaction is quenched with saturated sodium bicarbonate, washed with water and brine, dried over sodium sulfate, and concentrated under reduced pressure. The crude product is purified by silica gel column chromatography to yield the product as a white solid. The product is characterized by 1H NMR (600 MHz, CD3CN), 31P NMR (243 MHz, CD3CN) and LC-MS (ESI), with a measured [M+H]+ = 1182.6[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 2923115-67-5
-
性状 Solid
-
分子量 1182.52
-
分子式 C69H96N7O8P
-
Color White to off-white
-
SMILES
COC(C=C1)=CC=C1C(C2=CC=C(C=C2)OC)(C3=CC=CC=C3)OC[C@@H]4[C@@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](OCCCCCCCCCCCCCCCCCCCCCC)[C@H](N5C6=NC=NC(NC(C7=CC=CC=C7)=O)=C6N=C5)O4
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (84.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
-
データシート (272 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8457 mL | 4.2283 mL | 8.4565 mL | 21.1413 mL |
| 5 mM | 0.1691 mL | 0.8457 mL | 1.6913 mL | 4.2283 mL | |
| 10 mM | 0.0846 mL | 0.4228 mL | 0.8457 mL | 2.1141 mL | |
| 15 mM | 0.0564 mL | 0.2819 mL | 0.5638 mL | 1.4094 mL | |
| 20 mM | 0.0423 mL | 0.2114 mL | 0.4228 mL | 1.0571 mL | |
| 25 mM | 0.0338 mL | 0.1691 mL | 0.3383 mL | 0.8457 mL | |
| 30 mM | 0.0282 mL | 0.1409 mL | 0.2819 mL | 0.7047 mL | |
| 40 mM | 0.0211 mL | 0.1057 mL | 0.2114 mL | 0.5285 mL | |
| 50 mM | 0.0169 mL | 0.0846 mL | 0.1691 mL | 0.4228 mL | |
| 60 mM | 0.0141 mL | 0.0705 mL | 0.1409 mL | 0.3524 mL | |
| 80 mM | 0.0106 mL | 0.0529 mL | 0.1057 mL | 0.2643 mL |
DMTr-2'-O-C22-rA-3'-CE-Phosphoramidite 関連分類
HY-164583 関連分類
Powered by Bioz