Cat. No.: HY-W1058007

(E)-Caffeic acid phenethyl ester

(E)-Caffeic acid phenethyl ester is an inhibitor of iNOS and NF-κB. (E)-Caffeic acid phenethyl ester blocks the activation of iNOS by inhibiting NF-κB, and suppresses iNOS promoter activity at the NF-κB site. (E)-Caffeic acid phenethyl ester inhibits nitric oxide production in activated macrophages. (E)-Caffeic acid phenethyl ester is applicable to anti-inflammatory research.

For research use only. We do not sell to patients.

CAS No.: 115610-29-2
Formula: C₁₇H₁₆O₄
Molecular Weight:284.31

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

(E)-Caffeic acid phenethyl ester Chemical Structure

CAS No.: 115610-29-2

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

All NF-κB Isoforms :

All NO Synthase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	IC₅₀	26.8 μM Compound: 2; CAPE	Cytotoxicity against VHL-deficient human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay Cytotoxicity against VHL-deficient human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay	[PMID: 31260889]
786-0	IC₅₀	43.8 μM Compound: 2; CAPE	Cytotoxicity against VHL-positive human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay Cytotoxicity against VHL-positive human 786-0 cells assessed as reduction in cell viability incubated for 4 days by XTT assay	[PMID: 31260889]
A2780	EC₅₀	3.5 μM Compound: 17; CAPE	Cytotoxicity against human A2780 cells measured after 96 hrs by SRB assay Cytotoxicity against human A2780 cells measured after 96 hrs by SRB assay	[PMID: 31158743]
A549	EC₅₀	27.9 μM Compound: 14	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 12027739]
A549	IC₅₀	83.6 μM Compound: III-19	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 18952420]
A549	IC₅₀	26.8 μM Compound: 1, CAPE	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 20673727]
A549	IC₅₀	9.72 μM Compound: CAPE	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 26638042]
A549	IC₅₀	10 μM Compound: CAPE	Growth inhibition of human A549 cells Growth inhibition of human A549 cells	[PMID: 28814374]
A549	IC₅₀	80 μM Compound: 2; CAPE	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33210536]
B16-BL6	EC₅₀	6.79 μM Compound: 14	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 12027739]
B16-F10	IC₅₀	10.68 μM Compound: CAPE	Antiproliferative activity against mouse B16F10 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 26638042]
Bel-7402	IC₅₀	5.5 μM Compound: III-19	Cytotoxicity against human Bel7402 cells by MTT assay Cytotoxicity against human Bel7402 cells by MTT assay	[PMID: 18952420]
BeWo	IC₅₀	2.96 μM Compound: I-18	Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay	[PMID: 25160837]
BGC-823	IC₅₀	19.3 μM Compound: 1, CAPE	Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay	[PMID: 20673727]
BV-2	IC₅₀	6.4 μM Compound: 1, CAPE	Antineuroinflammatory activity in mouse BV2 cells assessed as reduction of LPS-induced nitric oxide production treated 3 hrs before LPS addition measured after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as reduction of LPS-induced nitric oxide production treated 3 hrs before LPS addition measured after 24 hrs by Griess assay	[PMID: 23726032]
BV-2	IC₅₀	6.4 μM Compound: 1, CAPE	Inhibition of nitric oxide production in LPS-stimulated mouse BV2 cells treated 3 hrs before LPS addition measured after 24 hrs by Griess assay Inhibition of nitric oxide production in LPS-stimulated mouse BV2 cells treated 3 hrs before LPS addition measured after 24 hrs by Griess assay	[PMID: 23870700]
BV-2	IC₅₀	6.4 μM Compound: 1, CAPE	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS challenge measured after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS challenge measured after 24 hrs by Griess assay	[PMID: 24697335]
Caco-2	IC₅₀	11.35 μM Compound: CAPE	Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay	[PMID: 26638042]
CNE	IC₅₀	54 μM Compound: 1, CAPE	Cytotoxicity against human CNE cells after 72 hrs by MTT assay Cytotoxicity against human CNE cells after 72 hrs by MTT assay	[PMID: 20673727]
DU-145	IC₅₀	16 μM Compound: CAPE	Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay	[PMID: 27979593]
FaDu	EC₅₀	12.1 μM Compound: 17; CAPE	Cytotoxicity against human FADU cells measured after 96 hrs by SRB assay Cytotoxicity against human FADU cells measured after 96 hrs by SRB assay	[PMID: 31158743]
HeLa	EC₅₀	2.36 μM Compound: 14	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 12027739]
HeLa	IC₅₀	0.068 μg/mL Compound: 9	Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA	[PMID: 19942440]
HeLa	IC₅₀	8.8 μM Compound: 1, CAPE	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 20673727]
HeLa	IC₅₀	37.98 μM Compound: I-18	Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay	[PMID: 25160837]
HeLa	IC₅₀	33 μM Compound: CAPE	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 27979593]
HepG2	IC₅₀	40.87 μM Compound: I-18	Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay	[PMID: 25160837]
HL-60	IC₅₀	9.8 μM Compound: 1, CAPE	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 20673727]
HL-60	IC₅₀	16.65 μM Compound: I-18	Antitumor activity against human HL60 cells assessed as inhibition of cell growth by MTT assay Antitumor activity against human HL60 cells assessed as inhibition of cell growth by MTT assay	[PMID: 25160837]
HT-1080	EC₅₀	9.5 μM Compound: 14	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 12027739]
HT-29	EC₅₀	30 μM Compound: 17; CAPE	Cytotoxicity against human HT-29 cells measured after 96 hrs by SRB assay Cytotoxicity against human HT-29 cells measured after 96 hrs by SRB assay	[PMID: 31158743]
HUVEC	EC₅₀	8 μM Compound: CAPE	Cytoprotective activity against H2O2-induced oxidative stress in HUVEC cells after 18 hrs by Cell-Titer Blue assay Cytoprotective activity against H2O2-induced oxidative stress in HUVEC cells after 18 hrs by Cell-Titer Blue assay	[PMID: 20598894]
IMR-32	IC₅₀	5 μM Compound: 2; CAPE	Cytotoxicity against human IMR-32 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human IMR-32 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33210536]
J774.1	IC₅₀	4.8 μM Compound: CAPE	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay	[PMID: 15974608]
JJN-3	IC₅₀	30 μM Compound: 2; CAPE	Growth inhibition of human JJN-3 cells after 48 hrs by PrestoBlue cell viability assay Growth inhibition of human JJN-3 cells after 48 hrs by PrestoBlue cell viability assay	[PMID: 33210536]
K562	IC₅₀	46 μM Compound: 1, CAPE	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 20673727]
KB	IC₅₀	45.2 μM Compound: 1, CAPE	Cytotoxicity against human KB cells after 72 hrs by MTT assay Cytotoxicity against human KB cells after 72 hrs by MTT assay	[PMID: 20673727]
KMM-1	IC₅₀	37.5 μM Compound: 2; CAPE	Growth inhibition of human KMM-1 cells after 48 hrs by PrestoBlue cell viability assay Growth inhibition of human KMM-1 cells after 48 hrs by PrestoBlue cell viability assay	[PMID: 33210536]
LNCaP	IC₅₀	6.2 μM Compound: 2, CAPE	Antiandrogenic activity in human LNCAP cells assessed as reduction in DHT-stimulated PSA release after 24 hrs by ELISA Antiandrogenic activity in human LNCAP cells assessed as reduction in DHT-stimulated PSA release after 24 hrs by ELISA	[PMID: 24080105]
LNCaP	IC₅₀	33.7 μM Compound: 2, CAPE	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay	[PMID: 24080105]
LS174T	IC₅₀	9.9 μM Compound: 1, CAPE	Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay	[PMID: 20673727]
Macrophage	IC₅₀	15 μM Compound: CAPE	Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay	[PMID: 15270564]
MCF7	IC₅₀	26.7 μM Compound: III-19	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 18952420]
MCF7	IC₅₀	28.8 μM Compound: CAPE	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 27979593]
MCF7	EC₅₀	12.1 μM Compound: 17; CAPE	Cytotoxicity against human MCF7 cells measured after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells measured after 96 hrs by SRB assay	[PMID: 31158743]
NIH3T3	EC₅₀	21.4 μM Compound: 17; CAPE	Cytotoxicity against mouse NIH/3T3 cells measured after 96 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells measured after 96 hrs by SRB assay	[PMID: 31158743]
PC-12	IC₅₀	2 μM Compound: CAPE	Neuroprotection activity in rat PC12 cells assessed as reduction in H2O2-induced cell death preincubated for 3 hrs followed by H2O2 addition and measured after 3 hrs by MTS assay Neuroprotection activity in rat PC12 cells assessed as reduction in H2O2-induced cell death preincubated for 3 hrs followed by H2O2 addition and measured after 3 hrs by MTS assay	[PMID: 30928712]
PC-3	IC₅₀	91 μM Compound: 1, CAPE	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 20673727]
PC-3	IC₅₀	51.4 μM Compound: 2, CAPE	Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue assay Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue assay	[PMID: 24080105]
PC-3	IC₅₀	10.15 μM Compound: CAPE	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 26638042]
Peritoneal macrophage	IC₅₀	4.31 μM Compound: 5; CAPE	Anti-inflammatory activity in ICR mouse peritoneal macrophages assessed inhibition of LPS-induced TNF-alpha production preincubated for 30 to 60 mins followed by LPS stimulation measured after 24 hrs by ELISA Anti-inflammatory activity in ICR mouse peritoneal macrophages assessed inhibition of LPS-induced TNF-alpha production preincubated for 30 to 60 mins followed by LPS stimulation measured after 24 hrs by ELISA	[PMID: 29202400]
RAW264.7	EC₅₀	4.518 μM Compound: Table 1, R23C1	Cytotoxicity against mouse RAW264.7 cells assessed as cell survival after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as cell survival after 24 hrs by MTT assay	[PMID: 18667320]
RAW264.7	EC₅₀	0.193 μM Compound: Table 1, R23C1	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay	[PMID: 18667320]
RAW264.7	IC₅₀	3.8 μM Compound: CAPE	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 10 mins prior to LPS-challenge measured after 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 10 mins prior to LPS-challenge measured after 18 hrs by Griess method	[PMID: 22831798]
RAW264.7	IC₅₀	9.3 μM Compound: CAPE	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method	[PMID: 28561586]
RAW264.7	IC₅₀	0.39 μM Compound: CAPE	Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of NO production pretreated for 1 hr followed by LPS addition and measured after 24 hrs by griess reagent based assay Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of NO production pretreated for 1 hr followed by LPS addition and measured after 24 hrs by griess reagent based assay	[PMID: 37976711]
RCC4	IC₅₀	14 μM Compound: 2; CAPE	Cytotoxicity against VHL-deficient human RCC4 cells assessed as reduction in cell viability incubated for 4 days by XTT assay Cytotoxicity against VHL-deficient human RCC4 cells assessed as reduction in cell viability incubated for 4 days by XTT assay	[PMID: 31260889]
RCC4/VHL	IC₅₀	29.1 μM Compound: 2; CAPE	Cytotoxicity against VHL-positive human RCC4/VHL cells assessed as reduction in cell viability incubated for 4 days by XTT assay Cytotoxicity against VHL-positive human RCC4/VHL cells assessed as reduction in cell viability incubated for 4 days by XTT assay	[PMID: 31260889]
SGC-7901	IC₅₀	14.26 μM Compound: I-18	Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay	[PMID: 25160837]
SiHa	IC₅₀	66.92 μM Compound: I-18	Antitumor activity against human SiHa cells assessed as inhibition of cell growth by MTT assay Antitumor activity against human SiHa cells assessed as inhibition of cell growth by MTT assay	[PMID: 25160837]
SW-1736	EC₅₀	4.7 μM Compound: 17; CAPE	Cytotoxicity against human SW1736 cells measured after 96 hrs by SRB assay Cytotoxicity against human SW1736 cells measured after 96 hrs by SRB assay	[PMID: 31158743]

In Vitro

(E)-Caffeic acid phenethyl ester (Compound 10) (2-200 μM; 24 h) potently inhibits NO production in LPS (HY-D1056)-activated mouse macrophage-like J774.1 cells, with an IC₅₀ of 5.44 μM, and shows no cytotoxicity at concentrations close to its IC₅₀^[1].
(E)-Caffeic acid phenethyl ester (1-100 μM; 24 h) potently inhibits NO production in LPS-activated mouse macrophage-like J774.1 cells, with an IC₅₀ of 7.64 μM^[1].
(E)-Caffeic acid phenethyl ester (1-100 μM; 24 h) shows no cytotoxicity toward LPS-treated mouse macrophage-like J774.1 cells at concentrations close to its NO inhibitory IC₅₀ (7.64 μM), but exhibits cytotoxicity at concentrations of 50 μM and 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

murine macrophage-like J774.1 cells
Concentration:

1, 10, 20, 50, 100 μM
Incubation Time:

24 h
Result:

Showed cell viabilities of 99.4%, 115%, 110%, 82.3%, and 67.4% respectively relative to the LPS-treated control at 1, 10, 20, 50, and 100 μM.
Exhibited cytotoxicity (cell viability < 90%) at 50 μM and 100 μM, but not at concentrations around the IC₅₀ value of 7.64 μM.

Chemical Information

CAS No. 115610-29-2
Molecular Weight 284.31
Formula C₁₇H₁₆O₄
SMILES

C(=C/C(OCCC1=CC=CC=C1)=O)\C2=CC(O)=C(O)C=C2
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Polyphenols
Initial Source
- Animals
Propolis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Nagaoka T, et al. Caffeic acid phenethyl ester (CAPE) analogues: potent nitric oxide inhibitors from the Netherlands propolis. Biological & pharmaceutical bulletin. 2003 Apr;26(4):487-91. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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(E)-Caffeic acid phenethyl ester

All NF-κB Isoforms

All NO Synthase Isoforms