(E/Z)-Platyphyllenone
(E/Z)-Platyphyllenone is a diarylheptanoid compound that can be found in Alnus viridis ssp. viridis. (E/Z)-Platyphyllenone inhibits the biosynthesis of violacein, but stimulates the production of AHL and AHQ quorum-sensing signal molecules as well as the synthesis of pyocyanin in Pseudomonas aeruginosa PAO1, and exhibits strong cytotoxicity against human lung fibroblasts. (E/Z)-Platyphyllenone can be used in studies on Pseudomonas aeruginosa.
For research use only. We do not sell to patients.
- CAS No.: 56973-65-0
- Formula: C19H20O3
- Molecular Weight:296.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
45 μM
Compound: 46
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Cytotoxicity against HEK293 cells after 16 hrs by alamar blue assay
Cytotoxicity against HEK293 cells after 16 hrs by alamar blue assay
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[PMID: 20004045] |
| HSC-T6 | IC50 |
34.7 μM
Compound: 1
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Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay
Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay
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[PMID: 21504848] |
| RAW264.7 | IC50 |
15.9 μM
Compound: 35
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
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[PMID: 33422907] |
| RAW264.7 | IC50 |
6.9 μM
Compound: 35
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 0.5 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 0.5 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
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[PMID: 33422907] |
| RAW264.7 | IC50 |
30.7 μM
Compound: 35
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Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability by MTT assay
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[PMID: 33422907] |
In Vitro
(E/Z)-Platyphyllenone (Compound 7) (0.5 MIC; 24 h) does not inhibit early biofilm formation in Pseudomonas aeruginosa PAO1[1].
(E/Z)-Platyphyllenone (10-15 µg/mL) reduces twitching and swimming motility in Pseudomonas aeruginosa PAO1[1].
(E/Z)-Platyphyllenone (50 µg/mL; 24 h) stimulates pyocyanin production to 127% of control levels in Pseudomonas aeruginosa PAO1[1].
(E/Z)-Platyphyllenone (100 µg per disc) inhibits violacein production in Chromobacterium violaceum CV026[1].
(E/Z)-Platyphyllenone (50 µg/mL; 24 h) stimulates 2-alkyl-4-quinolones production to 150% of control levels (50% relative increase) in Pseudomonas aeruginosa PAO1[1].
(E/Z)-Platyphyllenone (1-100 µg/mL; 48 h) exhibits concentration-dependent antiproliferative activity against MRC5 human lung fibroblasts, with an IC50 of 70 µg/mL, inhibiting proliferation by ~95% at both 50 µg/mL and 100 µg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MRC5 human lung fibroblast cells
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Concentration:1, 10, 50 and 100 µg/mL
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Incubation Time:48 h
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Result:Inhibited cell proliferation in a concentration-dependent manner.
Inhibited proliferation by about 95% at 50 µg/mL.
Inhibited proliferation by about 95% at 100 µg/mL.
Exhibited an IC50 value of 70 µg/mL for antiproliferative activity.
Chemical Information
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CAS No. 56973-65-0
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Molecular Weight 296.37
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Formula C19H20O3
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SMILES
O=C(C=CCCC1=CC=C(O)C=C1)CCC2=CC=C(O)C=C2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(E/Z)-Platyphyllenone Related Classifications
HY-N3043A Related Classifications
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