EGFR/Cytokine-IN-1 

EGFR/Cytokine-IN-1 is an EGFR (IC₅₀ = 0.03 μM) and Cytokine inhibitor (TNF-α, IC₅₀ = 3.1 μM; IL-6, IC₅₀ = 1.6 μM). EGFR/Cytokine-IN-1 shows potent anticancer activity on A549 and MCF7, respectively. EGFR/Cytokine-IN-1 significantly reduces IL-6 and TNF-α levels in A549 cells, and also shows promising ADMET profiles. EGFR/Cytokine-IN-1 can induce apoptosis in a dose-dependent manner. compound 5h can be used for the study of breast cancer and lung cancer^[1].

EGFR/Cytokine-IN-1 (Compound 5 h) (72 h) displays strong anticancer activity, with IC₅₀ values of 9.2 μM against A549 and 8.2 μM against MCF-7, and shows minimal cytotoxicity to MCF-10A (Non-Tumorigenic Cells)^[1].
EGFR/Cytokine-IN-1 (0.01 to 100 μM, 1 h) has a high binding affinity for EGFR (IC₅₀ = 0.03 μM)^[1].
EGFR/Cytokine-IN-1 shows the strongest dual inhibitory activity against TNF-α and IL-6, with IC₅₀ values of 3.1 μM and 1.6 μM, respectively^[1].
EGFR/Cytokine-IN-1 shows a significant buildup of cells in the G2/M phase and a clear reduction in the G0/G1 and S phases in A549 cells^[1].
EGFR/Cytokine-IN-1 shows noticeable apoptotic response, early apoptotic cells rose significantly to 51.75 %, while late apoptotic cells were at 30.40 %, and viable cells dropped to only 13.32 %; the total apoptosis rate reached 82.15 %, indicating a strong pro-apoptotic effect in A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

499.94

C₂₈H₂₂ClN₃O₄

N#CC1=C(C2=C(Cl)C=CC=C2)C=C(C3=CC=C(OC)C=C3)N=C1OCC(NC4=C(OC)C=CC=C4)=O

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Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lotfy EM, et al. Microwave-assisted one-pot green synthesis, ADMET profiling, DFT, and molecular docking of novel 3-cyano-2-pyridone derivatives as dual EGFR and cytokine (TNF-α, IL-6) inhibitors. Eur J Med Chem. 2025 Dec 15;300:118165.  [Content Brief]