Nuclease

T5 Exonuclease is a DNA exonuclease that has 5′-3′ exonuclease activity on both single- and double-stranded DNA. T5 Exonuclease also has single-strand endonuclease and 5′-flap endonuclease activity.

Ribonuclease T1 (animal free) (Rnase T1 (animal free)) (EC 4.6.1.24) is an endonuclease that specifically degrades single-stranded RNA. Ribonuclease T1 forms a nucleoside 2′, 3′-cyclic phosphate intermediate to cleave the phosphodiester bond between the 3′-guanosine residue and the 5′-OH group of the adjacent nucleoside to produce a 3′-GMP-terminated oligonucleotide. This product does not contain ingredients of animal origin.

DNA repair enzyme. Endonuclease IV has endonuclease activity at AP sites, 3' phosphodiesterase activity that can remove a variety of ligation-blocking lesions from the 3' end of DNA, endonuclease activity on oxidative DNA lesions, and 3' to 5' exonuclease activity.

TelN Protelomerase is a prokaryotic telomerase of bacteriophage N15. TelN Protelomerase is an enzyme with cleaving-joining activity, which is required for the formation of linear prophage DNA with closed ends in lysogenic bacteria. TelN Protelomerase cleaves both DNA strands and joins the resulting ends to form covalently closed hairpin structures.

Exonuclease III is a nuclease for specifically targeting double-stranded DNA (dsDNA). Exonuclease III is a DNA repair-associated nuclease with apurinic/apyrimidinic (AP)-endonuclease and 3'→5' exonuclease activities. Exonuclease III cleaves the ssDNA at 5'-bond of phosphodiester from 3' to 5' end by both exonuclease and endonuclease activities.

Endonuclease VIII is a DNA repair enzyme that excises oxidized pyrimidines from DNA. Endonuclease VIII is a bacterial DNA glycosylase/AP lyase that excises modified pyrimidines, including thymine glycol (Tg), uracil glycol, dihydrothymine, dihydrouracil (DHU).

RNase A (Bovine pancreatic RNase) is a widely used Endonuclease in DNA purification by specifically hydrolyzing cytosine or uracil residues of RNA. RNase A degrades the RNA in the RNA/DNA duplex. RNase A catalyses the breakdown of 3',5'-phosphodiester linkages of single stranded RNA. RNase A family members in organisms are tightly involved in various physiological and pathological processes including cell growth and development, proliferation, differentiation and migration. Dysregulation of RNase A activity or expression level is closely related to pancreatic, ovarian, bladder and thyroid cancer. RNase A has tumor cell-killing ability. RNase A, Bovine Pancreas (DNase & Protease Free) is RNase A derived from bovine pancreas and does not contain DNase or protease.

8-Oxo-dGDP phosphatase (EC 3.6.1.58) is a hydrolase that can catalyze multiple reactions, including reaction 1: convert 8-oxo-dGDP and H₂O into 8-oxo-dGMP, phosphate and H⁺; reaction 2: convert 8-oxo-GDP and H₂O into 8-oxo-GMP, phosphate and H⁺.

7-Methylguanosine nucleotidase (EC 3.1.3.91) is a hydrolase that can catalyze multiple reactions, including reaction 1: convert N(7)-methyl-GMP and H₂O into N(7)-methylguanosine and phosphate; reaction 2: convert CMP and H₂O into cytidine and phosphate.

7-Cyano-7-deazaguanine synthase (EC 6.3.4.20) is a ligase that can convert 7-carboxy-7-carbaguanine, NH₄⁺ and 2 ATP into 7-cyano-7-carbaguanine, 2 AMP, 2 diphosphate and 2 H⁺.

7-Carboxy-7-deazaguanine synthase (EC 4.3.99.3) is a lyase that can convert 6-carboxy-5,6,7,8-tetrahydropterin and H⁺ into 7-carboxy-7-carbaguanine and NH₄⁺.

7,8-Dihydroneopterin 2',3'-cyclic phosphate phosphodiesterase (EC 3.1.4.56) is a hydrolase that can catalyze multiple reactions, including reaction 1: convert 7,8-dihydroneopterin 2',3'-cyclic phosphate and H₂O into 7,8-dihydroneopterin 3'-phosphate and H⁺; reaction 2: convert 7,8-dihydroneopterin 2',3'-cyclic phosphate and H₂O into 7,8-dihydroneopterin 2'-phosphate and H⁺.

5-Methyldeoxycytidine-5'-phosphate kinase (EC 2.7.4.19) is a transferase that can convert 5-methyldeoxycytidine 5'-phosphate and ATP into 5-methyldeoxycytidine diphosphate and ADP.

5'-Deoxynucleotidase (EC 3.1.3.89) is a hydrolase that can convert a 2'-deoxyribonucleoside 5'-phosphate and H₂O into a 2'-deoxyribonucleoside and phosphate.

5'-Deoxyadenosine deaminase (EC 3.5.4.41) is a hydrolase that can convert 5'-deoxyadenosine, H₂O and H⁺ into 5'-deoxyinosine and NH₄⁺.

5'-(N7-methylguanosine 5'-triphospho)-[mRNA] hydrolase (EC 3.6.1.62) is a hydrolase that can convert a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA and H₂O into N(7)-methyl-GDP, a 5'-end phospho-ribonucleoside in mRNA and 2 H⁺.

5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] Di phosphatase (EC 3.6.1.59) is a hydrolase that can convert a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA and H₂O into a 5'-end diphospho-ribonucleoside in mRNA, N(7)-methyl-GMP and 2 H⁺.

5' to 3' Exodeoxyribonuclease (nucleoside 3'-phosphate-forming) (EC 3.1.12.1) is a hydrolase that can catalyze exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates.

3',5'-Nucleoside bisphosphate phosphatase (EC 3.1.3.97) is a phosphomonoesterase that hydrolyzes the 3'-phosphate group of nucleoside 3',5'-bisphosphates, yielding nucleoside 5'-phosphates and inorganic phosphate.

3'(2'),5'-Bisphosphate nucleotidase (EC 3.1.3.7) acts on 3'-phosphoadenylyl sulfate, and on the corresponding 2'-phosphates.