Nuclease

2'-Phosphotransferase (EC 2.7.1.160) catalyses the final step of tRNA splicing in the yeast Saccharomyces cerevisiae.

2'-Phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC 2.4.99.20) catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming NAADP+, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes.

2-Hydroxy-dATP diphosphatase (EC 3.6.1.56) hydrolyses oxidized purine nucleoside triphosphates such as 2-hydroxy-dATP, thereby preventing their misincorporation into DNA, and can also recognize 8-oxo-dGTP and 8-oxo-dATP.

2-Deoxyadenosine kinase (EC 2.7.1.76) is a transferase, an enzyme that transfers phosphorus-containing groups (phosphotransferases) using an alcohol group as an acceptor.

27S Pre-rRNA (guanosine2922-2'-O)-methyltransferase (EC 2.1.1.167) is formed in the later stages of 27S precursor rRNA maturation.

25S rRNA (adenine2142-N1)-Methyltransferase (EC 2.1.1.286) is a s-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine 2142 in 25S rRNA.

25S rRNA (uracil2843-N3)-Methyltransferase (EC 2.1.1.312), described from the yeast Saccharomyces cerevisiae, is involved in ribosome biogenesis.

25S rRNA (uracil2634-N3)-Methyltransferase (EC 2.1.1.313), described from the yeast Saccharomyces cerevisiae, is involved in ribosome biogenesis.

25S rRNA (cytosine2870-C5)-Methyltransferase (EC 2.1.1.310), found in eukaryotes, is specific for cytosine2870 of the 25S ribosomal RNA.

25S rRNA (cytosine2278-C5)-Methyltransferase (EC 2.1.1.311), found in eukaryotes, is specific for 25S cytosine2278.

25S rRNA (adenine645-N1)-methyltransferase (EC 2.1.1.287) is found in eukaryotes and is essential for ribosome biogenesis.

2'-5' Oligoadenylate synthase (EC 2.7.7.84) is activated by binding to double-stranded RNA. The resulting product binds to RNase L and activates RNase L, which then degrades the RNA. It also produces oligoadenylates with chain lengths of 2, 4, and 5.

23S rRNA Pseudouridine746 synthase (EC 5.4.99.29): rluA is the sole protein responsible for the in vivo formation of 23S RNA pseudouridine746.

23S rRNA Pseudouridine1911/1915/1917 synthase (EC 5.4.99.23) converts uridines at positions 1911, 1915, and 1917 of 23S rRNA to pseudouridines. These nucleotides are located in the functionally important helix-loop 69 of 23S rRNA.

23S rRNA Pseudouridine2605 synthase (EC 5.4.99.22): pseudouridine synthase RluB converts uridine2605 of 23S rRNA to pseudouridine.

23S rRNA Pseudouridine2604 synthase (EC 5.4.99.21) is not completely specific for uridine2604 and can, to a small extent, also react with uridine2605.

23S rRNA Pseudouridine2457 synthase (EC 5.4.99.20) modifies uridine2457 in a stem of 23S RNA in Escherichia coli.

23S rRNA (uridine2552-2'-O)-Methyltransferase (EC 2.1.1.166) catalyses the 2'-O-methylation of the universally conserved U2552 in the A loop of 23S rRNA.

23S rRNA (uridine2479-2'-O)-Methyltransferase (EC 2.1.1.208) can confer avilamycin resistance.

23S rRNA (uracil747-C5)-Methyltransferase (EC 2.1.1.189) specifically methylates uracil747 at C5 in 23S rRNA.