Eosin Y
Based on 3 publication(s) in Google Scholar
Eosin Y (Acid Red 87 free base) is a soluble acid red dye molecule. Eosin Y has a wide application in organic synthesis as a photoredox catalyst. Eosin Y can be used as a component of Eosin-Nigrosin Sperm Viability Staining Solution (HY-DY2022) to assess sperm viability and identify the integrity of the membrane structure and function of inactive sperm.
For research use only. We do not sell to patients.
- Purity : 99.42%
- CAS No.: 15086-94-9
- Formula: C20H8Br4O5
- Molecular Weight:647.89
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Eosin Y
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Customer Validation & Images
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Histological Imaging/Staining
Biological Activity
Description
In Vitro
Eosin Y is widely applied as a photoredox catalyst in organic synthesis[1].
Eosin Y finds application in cell staining, as a pH indicator, as an indicator in the analytical halide determination by Fajans and as a dye pigment, e.g. in lip sticks[1].
Eosin Y is the most widely used counterstain in routine staining of histological tissue sections (erythrocytes, collagen, epithelial cells, etc.)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 15086-94-9
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Appearance Solid
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Molecular Weight 647.89
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Formula C20H8Br4O5
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Color Yellow to orange
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(Br)C(O)=C4Br)C(Br)=C(O)C(Br)=C3)C5=C1C=CC=C5
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Synonyms
Acid Red 87 free base
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Sci Rep
Circ_0008043 promotes the metastasis of hepatocellular carcinoma by regulating the miR-661/PLEKHG4B axis. [Abstract]2025 Jul 8;15(1):24580. PMID: 40628993
Eosin Y purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 8;15(1):24580. [Abstract]
Represent images of H&E staining results of pulmonary metastatic focis. The sections were counterstained with Eosin Y for 3 min to visualize cytoplasmic structures. H&E staining showed fewer pulmonary metastatic lesions following circ_0008043 knockdown in HCC.
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Int J Chron Obstruct Pulmon Dis
Network Pharmacology Followed by Experimental Validation to Explore the Mechanism of Stigmasterol in Sangbaipi Decoction Regulating PI3K/Akt Signaling to Alleviate Acute Exacerbation of Chronic Obstructive Pulmonary Disease. [Abstract]2024 Aug 8:19:1819-1834. PMID: 39140079
Solvent & Solubility
In Vitro:
DMSO : 116.67 mg/mL (180.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Sperm Thawing for Functional Recovery
Sperm thawing for functional recovery is a post-cryopreservation procedure designed to restore and measure sperm motility, viability, membrane/acrosome integrity, DNA integrity, and usable motile sperm yield after freezing-induced injury; published human studies show that cryopreservation reduces motility and viability and can damage the plasmalemma, acrosome, tail, and DNA integrity. The experimental readout is generated by comparing post-thaw motility recovery, viability, acrosomal status, DNA integrity, and recovered motile sperm after thawing and optional sperm-selection steps; thawing at 40°C improved motility recovery compared with 20-37°C without significant differences in viability, ATP content, acrosomal status, or DNA integrity in one human donor-semen study.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (295 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.5435 mL | 7.7174 mL | 15.4347 mL | 38.5868 mL |
| 5 mM | 0.3087 mL | 1.5435 mL | 3.0869 mL | 7.7174 mL | |
| 10 mM | 0.1543 mL | 0.7717 mL | 1.5435 mL | 3.8587 mL | |
| 15 mM | 0.1029 mL | 0.5145 mL | 1.0290 mL | 2.5725 mL | |
| 20 mM | 0.0772 mL | 0.3859 mL | 0.7717 mL | 1.9293 mL | |
| 25 mM | 0.0617 mL | 0.3087 mL | 0.6174 mL | 1.5435 mL | |
| 30 mM | 0.0514 mL | 0.2572 mL | 0.5145 mL | 1.2862 mL | |
| 40 mM | 0.0386 mL | 0.1929 mL | 0.3859 mL | 0.9647 mL | |
| 50 mM | 0.0309 mL | 0.1543 mL | 0.3087 mL | 0.7717 mL | |
| 60 mM | 0.0257 mL | 0.1286 mL | 0.2572 mL | 0.6431 mL | |
| 80 mM | 0.0193 mL | 0.0965 mL | 0.1929 mL | 0.4823 mL | |
| 100 mM | 0.0154 mL | 0.0772 mL | 0.1543 mL | 0.3859 mL |
Eosin Y Related Classifications
HY-D0505 Related Classifications
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