Ethidium bromide (solution)
Based on 1 publication(s) in Google Scholar
Ethidium bromide (solution) is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Solvent and Concentration: Sterile water: 10 mg/Ml
For research use only. We do not sell to patients.
- CAS No.: 1239-45-8
- Formula: C21H20BrN3
- Molecular Weight:394.31
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Ethidium bromide (solution)
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Biological Activity
Description
Solvent and Concentration: Sterile water: 10 mg/Ml
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
0.1 μM
Compound: Ethidium
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Concentration required to inhibit 50% of human carcinoma A2780 cell lines
Concentration required to inhibit 50% of human carcinoma A2780 cell lines
|
[PMID: 15501053] |
| CH1 | IC50 |
0.4 μM
Compound: Ethidium
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Concentration required to inhibit 50% of human carcinoma CH1 cell lines
Concentration required to inhibit 50% of human carcinoma CH1 cell lines
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[PMID: 15501053] |
| MDA-MB-231 | IC50 |
2.33 μM
Compound: Ethidium bromide
|
Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA
Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA
|
[PMID: 25965778] |
| MGC-803 | IC50 |
2.1 μM
Compound: Ethidium bromide
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Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA
Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA
|
[PMID: 24119869] |
| MGC-803 | IC50 |
2.1 μM
Compound: Ethidium bromide
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Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA
|
[PMID: 25554922] |
| MGC-803 | IC50 |
2.18 μM
Compound: 2
|
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method
Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method
|
[PMID: 26883149] |
| MGC-803 | IC50 |
2.39 μM
Compound: Ethidium bromide
|
Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
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[PMID: 23265905] |
| MGC-803 | IC50 |
2.47 μM
Compound: Ethidium bromide
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Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay
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[PMID: 22405648] |
| NIH3T3 | IC50 |
134.87 μM
Compound: Ethidium bromide
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Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay
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[PMID: 24286761] |
| SGC-7901 | IC50 |
2.5 μM
Compound: Ethidium bromide
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Inhibition of telomerase in human SGC7901 cells by TRAP assay
Inhibition of telomerase in human SGC7901 cells by TRAP assay
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[PMID: 20800480] |
| SGC-7901 | IC50 |
2.5 μM
Compound: Ethidium bromide
|
Inhibition of telomerase in human SGC7901 cells by TRAP assay
Inhibition of telomerase in human SGC7901 cells by TRAP assay
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[PMID: 20947362] |
| SGC-7901 | IC50 |
2.6 μM
Compound: Ethidium bromide
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Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP
Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP
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[PMID: 21486698] |
| SGC-7901 | IC50 |
2.6 μM
Compound: Ethidium bromide
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Inhibition of telomerase in human SGC7901 cells by TRAP assay
Inhibition of telomerase in human SGC7901 cells by TRAP assay
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[PMID: 23312949] |
| SK-OV-3 | IC50 |
4.5 μM
Compound: Ethidium
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Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines
Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines
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[PMID: 15501053] |
| SW 1116 | IC50 |
2.71 μM
Compound: Ethidium bromide
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Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay
Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay
|
[PMID: 24286761] |
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The preparation of agarose gel:
1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.
2. Mix the agarose powder with 1 X TAE/TBE.
3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).
4. Make the solution cool down before solidification.
5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/mL, and require 5 μL stock/100 mL gel).
6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.
CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1239-45-8
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Appearance Liquid (Density: 1.3739 g/cm3)
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Molecular Weight 394.31
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Formula C21H20BrN3
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Color Brown to red
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SMILES
CC[N+]1=C(C2=CC(N)=CC=C2C3=C1C=C(C=C3)N)C4=CC=CC=C4.[Br-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Biol Reprod
O-GlcNAcylation of p66Shc drives senescence associated secretory phenotype via mitochondrial DNA release in gestational diabetes mellitus†. [Abstract]2026 May 11:ioag090. PMID: 42113624
Protocols
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Agarose gel electrophoresis (200 bp-5 kb)
Agarose gel electrophoresis is a molecular biology technique that uses agarose gel as a support medium to separate DNA fragments through a combination of molecular sieving and charge effects.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ethidium bromide (solution) Related Classifications
HY-DY1050 Related Classifications
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