Fluorescein-PEG4-NHS ester

Cat. No.: HY-D2329 Purity: 95.22%: Data Sheet
COA

SDS
Handling Instructions
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Fluorescein-PEG4-NHS ester is a colorimetric probe formed by the reaction of adipic dihydrazide and fluorescein isothiocyanate.

For research use only. We do not sell to patients.

Fluorescein-PEG4-NHS ester Chemical Structure

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Biological Activity
Purity & Documentation
References
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Description

Fluorescein-PEG4-NHS ester is a colorimetric probe formed by the reaction of adipic dihydrazide and fluorescein isothiocyanate^[1].

In Vitro

Fluorescein-PEG4-NHS ester is a colorimetric probe formed by the reaction of adipic dihydrazide and fluorescein isothiocyanate^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

751.76

Formula

C₃₆H₃₇N₃O₁₃S

Appearance

Solid

Color

Yellow to orange

SMILES

S=C(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)NCCOCCOCCOCCOCCC(ON6C(CCC6=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (66.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3302 mL	6.6511 mL	13.3021 mL
5 mM	0.2660 mL	1.3302 mL	2.6604 mL
10 mM	0.1330 mL	0.6651 mL	1.3302 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.22%

Select Batch:

Data Sheet (269 KB) SDS (251 KB)

COA (248 KB) HNMR (318 KB) RP-HPLC (266 KB) LCMS (235 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014;133:54-59.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3302 mL	6.6511 mL	13.3021 mL	33.2553 mL
	5 mM	0.2660 mL	1.3302 mL	2.6604 mL	6.6511 mL
	10 mM	0.1330 mL	0.6651 mL	1.3302 mL	3.3255 mL
	15 mM	0.0887 mL	0.4434 mL	0.8868 mL	2.2170 mL
	20 mM	0.0665 mL	0.3326 mL	0.6651 mL	1.6628 mL
	25 mM	0.0532 mL	0.2660 mL	0.5321 mL	1.3302 mL
	30 mM	0.0443 mL	0.2217 mL	0.4434 mL	1.1085 mL
	40 mM	0.0333 mL	0.1663 mL	0.3326 mL	0.8314 mL
	50 mM	0.0266 mL	0.1330 mL	0.2660 mL	0.6651 mL
	60 mM	0.0222 mL	0.1109 mL	0.2217 mL	0.5543 mL