FM-red
Based on 1 Customer Validation
FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols. FM-red can be used to image protein sulfhydryl groups in live cells and in vivo. FM-red also could be used to measure of the redox states of thioredoxin (Trx).
For research use only. We do not sell to patients.
- Purity: 98.81%
- Formula: C41H46ClN5O9
- Molecular Weight:788.29
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
FM-red (10 μM; 30 min) is suitable for imaging protein thiols in HeLa cells[1].
FM-red (20 μM; 48 h) has no significant cytotoxicity in HeLa cells[1].
FM-red possesses a long emission wavelength (~655 nm) and fast response (~10 min) by binding to protein thiols[1].
FM-red exhibits time- and concentration-dependent fluorescence response to bovine serum albumin (BSA)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 788.29
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Formula C41H46ClN5O9
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Color Light brown to brown
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SMILES
CN(C1=CC=C(C2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=C(C=CC=C4)C(N5CCC(CC5)NC(CCCN6C(C=CC6=O)=O)=O)=O)=C2)C=C1)C.O=Cl(=O)([O-])=O
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Synonyms
PSH-red
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 50 mg/mL (63.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2686 mL | 6.3428 mL | 12.6857 mL | 31.7142 mL |
| 5 mM | 0.2537 mL | 1.2686 mL | 2.5371 mL | 6.3428 mL | |
| 10 mM | 0.1269 mL | 0.6343 mL | 1.2686 mL | 3.1714 mL | |
| 15 mM | 0.0846 mL | 0.4229 mL | 0.8457 mL | 2.1143 mL | |
| 20 mM | 0.0634 mL | 0.3171 mL | 0.6343 mL | 1.5857 mL | |
| 25 mM | 0.0507 mL | 0.2537 mL | 0.5074 mL | 1.2686 mL | |
| 30 mM | 0.0423 mL | 0.2114 mL | 0.4229 mL | 1.0571 mL | |
| 40 mM | 0.0317 mL | 0.1586 mL | 0.3171 mL | 0.7929 mL | |
| 50 mM | 0.0254 mL | 0.1269 mL | 0.2537 mL | 0.6343 mL | |
| 60 mM | 0.0211 mL | 0.1057 mL | 0.2114 mL | 0.5286 mL |
FM-red Related Classifications
HY-D1260 Related Classifications
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