FM-red  (Synonyms: PSH-red)

FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols. FM-red can be used to image protein sulfhydryl groups in live cells and in vivo. FM-red also could be used to measure of the redox states of thioredoxin (Trx)^[1].

FM-red (10 μM; 30 min) is suitable for imaging protein thiols in HeLa cells^[1].
FM-red (20 μM; 48 h) has no significant cytotoxicity in HeLa cells^[1].
FM-red possesses a long emission wavelength (~655 nm) and fast response (~10 min) by binding to protein thiols^[1].
FM-red exhibits time- and concentration-dependent fluorescence response to bovine serum albumin (BSA)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FM-red (10 μM; 30 min) could be used to image protein thiols in zebrafishes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

788.29

C₄₁H₄₆ClN₅O₉

Solid

Brown to black

CN(C1=CC=C(C2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=C(C=CC=C4)C(N5CCC(CC5)NC(CCCN6C(C=CC6=O)=O)=O)=O)=C2)C=C1)C.O=Cl(=O)([O-])=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Hu G, et, al. Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe. J Mater Chem B. 2019 Apr 28;7(16):2696-2702.  [Content Brief]