Br-DAPI
Based on 3 publication(s) in Google Scholar
Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells. Storage: Keep away from light.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.0%
- No. CAS: 2387906-44-5
- Fòrmula: C16H14BrN5
- Peso molecular:356.22
-
Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Br-DAPI
More
Actividad biológica
Descripciòn
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | CC50 |
>64 μM
Compound: Br-DAPI
|
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay
|
[PMID: 36480920] |
| MCF7 | CC50 |
7.6 μM
Compound: Br-DAPI
|
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay
|
[PMID: 36480920] |
In Vitro
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of DAPI working solution
1.1 Preparation of the stock solution
Dissolve 1 mg DAPI in 1 mL ddH2O to obtain 1 mg/mL of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of DAPI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 ug/mL of working solution.
Note: Please adjust the concentration of DAPI working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.
Precautions 1. Please adjust the concentration of DAPI working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
No. CAS 2387906-44-5
-
Appearance Solid
-
Peso molecular 356.22
-
Fòrmula C16H14BrN5
-
Color Light yellow to yellow
-
SMILES
N=C(C1=CC2=C(C=C1)C(Br)=C(C3=CC=C(C(N)=N)C=C3)N2)N
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
-
Journal Impact Factor
-
Most Recent
-
Pharmacol Res
3'-epi-12β-hydroxyfroside-mediated autophagy degradation of RIPK1/RIPK3 necrosomes leads to anergy of immunogenic cell death in triple-negative breast cancer cells. [Abstract]2023 Jan:187:106613. PMID: 36535569 -
Cell Transplant
Dexamethasone Induces Senescence-Associated Changes in Trabecular Meshwork Cells by Increasing ROS Levels Via the TGFβ/Smad3-NOX4 Axis. [Abstract]2023 Jan-Dec:32:9636897231177356. PMID: 37265069 -
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (280.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
-
Nuclear DNA counterstaining and nuclear morphology staining
Nuclear DNA counterstaining uses DNA-binding fluorescent dyes to visualize nuclei and chromatin so that nuclei can be located, counted, segmented, and evaluated for morphology; Hoechst 33342, DAPI, propidium iodide, and DRAQ5 are commonly reported nuclear stains, while live-cell DNA labeling is better supported for Hoechst dyes and DRAQ5 than for propidium iodide in intact viable cells. Nuclear morphology staining can detect apoptosis-associated nuclear changes, including chromatin condensation, nuclear shrinkage, nuclear fragmentation, reduced nuclear area/perimeter/axis length, and increased nuclear fluorescence intensity; these morphology readouts have been compared with apoptosis markers such as TUNEL and caspase-3 immunofluorescence.
Pureza y Documentación
-
Ficha de datos (272 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8073 mL | 14.0363 mL | 28.0725 mL | 70.1813 mL |
| 5 mM | 0.5615 mL | 2.8073 mL | 5.6145 mL | 14.0363 mL | |
| 10 mM | 0.2807 mL | 1.4036 mL | 2.8073 mL | 7.0181 mL | |
| 15 mM | 0.1872 mL | 0.9358 mL | 1.8715 mL | 4.6788 mL | |
| 20 mM | 0.1404 mL | 0.7018 mL | 1.4036 mL | 3.5091 mL | |
| 25 mM | 0.1123 mL | 0.5615 mL | 1.1229 mL | 2.8073 mL | |
| 30 mM | 0.0936 mL | 0.4679 mL | 0.9358 mL | 2.3394 mL | |
| 40 mM | 0.0702 mL | 0.3509 mL | 0.7018 mL | 1.7545 mL | |
| 50 mM | 0.0561 mL | 0.2807 mL | 0.5615 mL | 1.4036 mL | |
| 60 mM | 0.0468 mL | 0.2339 mL | 0.4679 mL | 1.1697 mL | |
| 80 mM | 0.0351 mL | 0.1755 mL | 0.3509 mL | 0.8773 mL | |
| 100 mM | 0.0281 mL | 0.1404 mL | 0.2807 mL | 0.7018 mL |
Br-DAPI Related Classifications
HY-D1396 Related Classifications
Powered by Bioz