Cat. No.: HY-113329

Guanidinoethyl sulfonate

Name: Guanidinoethyl sulfonate
Brand: MedChemExpress
SKU: HY-113329
Price: 50.00 USD
Availability: InStock
Rating: 5 (5 reviews)

(Synonyms: Taurocyamine)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Guanidinoethyl sulfonate (Taurocyamine) is an orally available, blood-brain permeable competitive inhibitor of taurine transporters and a competitive antagonist of glycine receptors (GlyR) (IC₅₀=565 μM). Guanidinoethyl sulfonate has both weak agonist and antagonist effects on GABA_A receptors. Guanidinoethyl sulfonate inhibits taurine transmembrane transport and competitively binds to the GlyR ligand binding domain, thereby blocking glycine-mediated chloride influx, and may regulate brain pH to exert neuroprotective effects. Guanidinoethyl sulfonate can be used for neuroprotection studies of ischemic brain injury.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 543-18-0
Formula: C₃H₉N₃O₃S
Molecular Weight:167.19

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Guanidinoethyl sulfonate Chemical Structure

CAS No.: 543-18-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Electrophysiological experiments (Glycine receptor activity):
Guanidinoethyl sulfonate (0.5 mM; transient) competitively antagonizes glycine-induced currents in mouse striatal neurons, shifting the glycine dose-response curve to the right, with the EC₅₀ increasing from 62 μM (control group) to 154 μM (treated group)^[1].
Electrophysiological experiments (GABA receptor activity):
Guanidinoethyl sulfonate (1 mM; transient) exhibits both weak agonist and antagonist effects on GABA_A receptors in mouse striatal neurons^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Mouse striatal giant aspiny neurons (GAN) and medium spiny neurons (MSN)
Concentration:

0.5 mM, 1 mM
Incubation Time:
Result:

At 0.5 mM, shifted the glycine dose-response curve rightward without affecting maximal response, increasing EC₅₀ from 62 μM to 154 μM and decreasing the Hill coefficient from 2.5 to 1.3.
At 1 mM, reduced glycine (100 μM)-evoked currents to 36 ± 2.7% in GAN and 35 ± 3.9% in MSN, with voltage-independent block confirmed by unchanged reversal potentials near chloride equilibrium potential.

In Vivo

Forebrain ischemia model:
Guanidinoethyl sulfonate (625 mg/kg; intraperitoneal injection; once a day; 2 weeks) pretreatment in adult male gerbils significantly increases the survival rate of hippocampal CA1 neurons after ischemia and alleviates delayed neuronal death^[2].
Pregnancy model:
Guanidinoethyl sulfonate (1% drinking water; free drinking; gestational day 11-21) causes a significant decrease in taurine concentrations in the fetus' body, liver, brain and placenta (32%-87%), reduces fetal body weight and organ weights, and increases maternal urinary taurine excretion in the Wistar pregnant rat model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male gerbils (70 g), forebrain ischemia-reperfusion model^[2]
Dosage:

625 mg/kg, dissolved in distilled water (neutralized to pH 7.0 with NaOH)
Administration:

Intraperitoneal injection, daily for 2 weeks, followed by 5-minute bilateral carotid artery occlusion and 7-day reperfusion
Result:

Significantly increased the number of surviving CA1 neurons (61.1 cells/mm) compared to saline-treated ischemic controls (17.75 cells/mm).
Decreased brain taurine levels, while increased intracellular pH, indicating a protective effect against ischemic neuronal death without affecting baseline neuronal density in non-ischemic controls.

Animal Model:

Pregnant Wistar rats (initial weight 190-230 g), gestational taurine depletion model^[3]
Dosage:

1% guanidinoethyl sulfonate (GES) in drinking water
Administration:

Free access to GES-containing water from gestational day 11 to 21 (10 days total)
Result:

Led to significant taurine depletion in fetal tissues (54% in whole body, 37% in liver, 87% in brain, 32% in placenta) and maternal tissues (33% in liver, 32% in brain, 46% in plasma).
Fetal wet weights of whole body, liver, brain, and placenta were reduced by 17%, 24%, 5%, and 13%, respectively, compared to controls.
Maternal urinary taurine excretion increased significantly throughout the treatment period, with no changes in maternal organ weights or food/water intake.

Chemical Information

CAS No. 543-18-0
Appearance Solid
Molecular Weight 167.19
Formula C₃H₉N₃O₃S
Color White to off-white
SMILES

N=C(NCCS(=O)(O)=O)N
Synonyms

Taurocyamine
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

All In Vivo Efficacy StudyCell Imaging/StainingBio/Physico-chemical Assay

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Journal Impact Factor

Nat Commun

Molecular basis of human taurine transporter uptake and inhibition. [Abstract]

2025 Aug 11;16(1):7394. PMID: 40789850
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
Invest Ophthalmol Vis Sci

SLC6A6-Mediated Taurine Uptake Sustains Corneal Epithelial Stem/Progenitor Cell Function to Counteract Age-Related Dysfunction. [Abstract]

2025 Jun 2;66(6):25. PMID: 40478558

Guanidinoethyl sulfonate purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):25. [Abstract]

The corneal epithelia of mice (3-month-old males) treated with oral Guanidinoethyl sulfonate, oral and topical Guanidinoethyl sulfonate, or control were stained with fluorescein sodium at 0, 24, 48, and 60 hours after wounding (left panel), and the corneal wound closure was presented as the percentage of the original wound.(1% Guanidinoethyl sulfonate was added to water 7 days prior to corneal epithelial debridement. Subsequently, using 100-mM Guanidinoethyl sulfonate eye drops or PBS five times daily for 3 days post-wounding.)

Guanidinoethyl sulfonate purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):25. [Abstract]

Colony formation (left panel) and number (right panel) in Guanidinoethyl sulfonate (0-40 mM)-treated TKE2 cells.

Guanidinoethyl sulfonate purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):25. [Abstract]

PCA analysis of TKE2 cells treated with Guanidinoethyl sulfonate versus control (CON) groups.

Guanidinoethyl sulfonate purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):25. [Abstract]

KEGG analysis of upregulated/downregulated pathways in Guanidinoethyl sulfonate-treated TKE2 cells.
Metabolites

2025 Dec 22;16(1):6.

Solvent & Solubility

In Vitro:

H₂O : 41.67 mg/mL (249.24 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.9812 mL

29.9061 mL

59.8122 mL

5 mM

1.1962 mL

5.9812 mL

11.9624 mL

10 mM

0.5981 mL

2.9906 mL

5.9812 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 25 mg/mL (149.53 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sergeeva OA, et al. Guanidinoethyl sulphonate is a glycine receptor antagonist in striatum. Br J Pharmacol. 2002 Nov;137(6):855-60. [Content Brief]

[2]. Igarashi H, et al. Guanidinoethane sulfate is neuroprotective towards delayed CA1 neuronal death in gerbils. Life Sci. 1995;56(14):1201-6. [Content Brief]

[3]. K Ejiri, et al. Effect of guanidinoethyl sulfonate on taurine concentrations and fetal growth in pregnant rats. Biol Neonate. 1987;51(4):234-40. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	5.9812 mL	29.9061 mL	59.8122 mL	149.5305 mL
	5 mM	1.1962 mL	5.9812 mL	11.9624 mL	29.9061 mL
	10 mM	0.5981 mL	2.9906 mL	5.9812 mL	14.9530 mL
	15 mM	0.3987 mL	1.9937 mL	3.9875 mL	9.9687 mL
	20 mM	0.2991 mL	1.4953 mL	2.9906 mL	7.4765 mL
	25 mM	0.2392 mL	1.1962 mL	2.3925 mL	5.9812 mL
	30 mM	0.1994 mL	0.9969 mL	1.9937 mL	4.9843 mL
	40 mM	0.1495 mL	0.7477 mL	1.4953 mL	3.7383 mL
	50 mM	0.1196 mL	0.5981 mL	1.1962 mL	2.9906 mL
	60 mM	0.0997 mL	0.4984 mL	0.9969 mL	2.4922 mL
	80 mM	0.0748 mL	0.3738 mL	0.7477 mL	1.8691 mL
	100 mM	0.0598 mL	0.2991 mL	0.5981 mL	1.4953 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

Guanidinoethyl sulfonate

5 Cited Publications

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Guanidinoethyl sulfonate

Hypotaurine

Taurine

β-Alanine