1. Metabolic Enzyme/Protease
    Apoptosis
  2. Carbonic Anhydrase
    Apoptosis
  3. hCAIX/XII-IN-5

hCAIX/XII-IN-5 

Cat. No.: HY-150695
Handling Instructions

hCAIX/XII-IN-5 (Coumarin 9a) a carbonic anhydrase (CA) inhibitor, and exhibits excellent hCA IX/XII selectivity (Ki=93.9 and 85.7 nM, respectively) over hCA I and hCA II. hCAIX/XII-IN-5 shows anti-proliferative activities to cancer cells. hCAIX/XII-IN-5 can delay cell cycle and induce apoptosis.

For research use only. We do not sell to patients.

hCAIX/XII-IN-5 Chemical Structure

hCAIX/XII-IN-5 Chemical Structure

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

hCAIX/XII-IN-5 (Coumarin 9a) a carbonic anhydrase (CA) inhibitor, and exhibits excellent hCA IX/XII selectivity (Ki=93.9 and 85.7 nM, respectively) over hCA I and hCA II. hCAIX/XII-IN-5 shows anti-proliferative activities to cancer cells. hCAIX/XII-IN-5 can delay cell cycle and induce apoptosis[1].

IC50 & Target

hCA XII

85.7 nM (Ki)

hCA IX

93.9 nM (Ki)

In Vitro

hCAIX/XII-IN-5 (2.36-32.17 μM) treatment shows both anti-proliferative activities to MCF-7 and PANC-1 cells under the normoxic and hypoxic condition[1].
hCAIX/XII-IN-5 (2.69 μM; 24 h) treatment shows accumulation of MCF-7 cells in the Sub-G1 phase and cell arrest in G2-M phase[1].
hCAIX/XII-IN-5 (2.69 μM; 24 h) treatment increases the percent of annexin V-FITC-positive apoptotic cells, including both the early and late apoptotic cells (UR+LR) [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: MCF-7 and PANC-1 cells
Concentration: 2.36-32.17 μM
Incubation Time:
Result: Exerted anti-proliferative activities to MCF-7 and PANC-1 cells (IC50=2.69 μM and 32.17 μM, respectively) under the normoxic condition, and moderate activitits against both MCF-7 and PANC-1 cells (IC50=16.36 μM and 11.78 μM, respectively) under the hypoxic condition.

Cell Cycle Analysis[1]

Cell Line: MCF-7 cells
Concentration: 2.69 μM
Incubation Time: 24 hours
Result: Resulted in a significant increase in the percentage of cells at Sub-G1 by 14-folds with significant increase in cell percentage in the G1-M phase by 2.3-folds compared to the control.

Apoptosis Analysis[1]

Cell Line: MCF-7 cells
Concentration: 2.69 μM
Incubation Time: 24 hours
Result: Resulted in a significant increase in the percent of annexin V-FITC-positive apoptotic cells, including both the early (from 0.61% to 6.77%) and late apoptotic (from 0.27% to 14.84%) cells (UR+LR), about 11- and 55-fold increase, respectively, as compared with the control.
Molecular Weight

291.30

Formula

C18H13NO3

SMILES

O=C1C(C(/C=C/NC2=CC=CC=C2)=O)=CC3=C(O1)C=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
hCAIX/XII-IN-5
Cat. No.:
HY-150695
Quantity:
MCE Japan Authorized Agent: