Cat. No.: HY-N1422

Hecogenin

Name: Hecogenin
Brand: MedChemExpress
SKU: HY-N1422
Price: 40.00 USD
Availability: InStock
Rating: 5 (1 reviews)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Hecogenin is a steroid saponin isolated from Agave sisalana and is a selective inhibitor of human UDP-glucuronosyltransferases. Hecogenin has a wide spectrum of pharmacological activities, including anti-inflammatory, antifungal and gastroprotective effects.

For research use only. We do not sell to patients.

Purity : 99.96%
CAS No.: 467-55-0
Formula: C₂₇H₄₂O₄
Molecular Weight:430.62

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Hecogenin Chemical Structure

CAS No.: 467-55-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Publications Citing Use of MedChemExpress (MCE) Hecogenin

Curr Issues Mol Biol. 2025 Sep 26;47(10):798. [Abstract]

Customer Validation & Images

Cell Proliferation/Viability AssayCell Migration/Invasion AssayWBRT-PCR

Cell Proliferation/Viability Assay
Cell Migration/Invasion Assay
WB
RT-PCR

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Protocols (1)
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human UDP-glucuronosyltransferases^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>40 μM Compound: Hecogenin	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33735799]
BT-474	IC₅₀	38.2 μM Compound: 1	Antiproliferative activity against human BT474 cells after 72 hrs by MTT assay Antiproliferative activity against human BT474 cells after 72 hrs by MTT assay	[PMID: 29066046]
HT-29	IC₅₀	>40 μM Compound: Hecogenin	Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33735799]
HUVEC	IC₅₀	>100 μM Compound: Hecogenin	Cytotoxicity against human HUVEC cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HUVEC cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33735799]
MCF7	IC₅₀	28.7 μM Compound: Hecogenin	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33735799]
MCF7	IC₅₀	31 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 29066046]
MDA-MB-231	IC₅₀	28.7 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 29066046]
MDA-MB-231	IC₅₀	31.21 μM Compound: Hecogenin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 33735799]
MDA-MB-231	IC₅₀	37.1 μM Compound: 1	Antiinvasive activity against human MDA-MB-231 cells after 24 hrs by cell invasion assay Antiinvasive activity against human MDA-MB-231 cells after 24 hrs by cell invasion assay	[PMID: 29066046]
MDA-MB-468	IC₅₀	35.2 μM Compound: 1	Antiproliferative activity against human MDA-MB-468 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells after 72 hrs by MTT assay	[PMID: 29066046]
SK-BR-3	IC₅₀	33.3 μM Compound: 1	Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT assay Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 29066046]
T47D	IC₅₀	36.6 μM Compound: 1	Antiproliferative activity against human T47D cells after 72 hrs by MTT assay Antiproliferative activity against human T47D cells after 72 hrs by MTT assay	[PMID: 29066046]

In Vivo

Hecogenin (3.1-90 mg/kg; oral administration; for 15 hours; male Swiss mice) acutely administered, before ethanol, exhibits a potent gastroprotective effect. The Hecogenin pretreatment normalizes GSH levels and significantly reduces lipid peroxidation and nitrite levels in the stomach, as evaluated by the ethanol-induced gastric lesion model. Hecogenin also decreases MPO release and significantly protects the gastric mucosa^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Swiss mice (20-30 g) with ethanol^[1]
Dosage:

3.1 mg/kg, 7.5 mg/kg, 15 mg/kg, 30 mg/kg, 60 mg/kg and 90 mg/kg
Administration:

Oral administration; for 15 hours
Result:

Normalized GSH levels and significantly reduced lipid peroxidation and nitrite levels in the stomach, as evaluated by the ethanol-induced gastric lesion model. Decreased MPO release and significantly protected the gastric mucosa.

Chemical Information

CAS No. 467-55-0
Appearance Solid
Molecular Weight 430.62
Formula C₂₇H₄₂O₄
Color White to off-white
SMILES

[H][C@]1(O[C@@]2(OC[C@H](C)CC2)[C@@H](C)[C@@]1([C@]34C)[H])C[C@@]3([H])[C@]5([H])CC[C@@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC4=O
Structure Classification
- Steroids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

All Cell Proliferation/Viability AssayCell Migration/Invasion AssayWBRT-PCR

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Journal Impact Factor

Curr Issues Mol Biol

Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays. [Abstract]

2025 Sep 26;47(10):798. PMID: 41150746

Hecogenin purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2025 Sep 26;47(10):798. [Abstract]

Effect of different concentrations of Hecogenin (1. 20, 40, 80, 160 μM) on CFs viability.

Hecogenin purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2025 Sep 26;47(10):798. [Abstract]

Representative images from the Transwell assay showing the migration of CFs treated with MG-EWE, Laurolitsine, and Hecogenin (1 μM).

Hecogenin purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2025 Sep 26;47(10):798. [Abstract]

Electrophoretic bands of Collagen I, Collagen III, α-SMA, p-ADRB2, ADRB2, p-JNK, JNK, p-c-Jun, c-Jun, and IL-6 protein expression treated with Hecogenin (1 μM).

Hecogenin purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2025 Sep 26;47(10):798. [Abstract]

Expression level of IL-6 mRMA in CFs of rats treated with Hecogenin (1 μM).

Solvent & Solubility

In Vitro:

DMF : 16.67 mg/mL (38.71 mM; ultrasonic and warming and heat to 60°C)

H₂O : < 0.1 mg/mL (insoluble)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3222 mL

11.6112 mL

23.2223 mL

5 mM

0.4644 mL

2.3222 mL

4.6445 mL

10 mM

0.2322 mL

1.1611 mL

2.3222 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

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Protocols

All Signal Pathway Related

Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Santos Cerqueira G, et al. Effects of hecogenin and its possible mechanism of action on experimental models of gastric ulcer in mice. Eur J Pharmacol. 2012 May 15;683(1-3):260-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMF	1 mM	2.3222 mL	11.6112 mL	23.2223 mL	58.0558 mL
	5 mM	0.4644 mL	2.3222 mL	4.6445 mL	11.6112 mL
	10 mM	0.2322 mL	1.1611 mL	2.3222 mL	5.8056 mL
	15 mM	0.1548 mL	0.7741 mL	1.5482 mL	3.8704 mL
	20 mM	0.1161 mL	0.5806 mL	1.1611 mL	2.9028 mL
	25 mM	0.0929 mL	0.4644 mL	0.9289 mL	2.3222 mL
	30 mM	0.0774 mL	0.3870 mL	0.7741 mL	1.9352 mL

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Hecogenin

Publications Citing Use of MedChemExpress (MCE) Hecogenin