IDO1/2-IN-1 hydrochloride

Cat. No.: HY-145280A: FAQs
Data Sheet Handling Instructions

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IDO1/2-IN-1 hydrochloride (compound 4t) is the first potent IDO1/IDO2 dual inhibitor with IC₅₀s of 28 nM and 144 nM for IDO1 and IDO2, respectively. IDO1/2-IN-1 hydrochloride exhibits antitumor activies. Orally active.

For research use only. We do not sell to patients.

IDO1/2-IN-1 hydrochloride Chemical Structure

CAS No. : 2310286-60-1

Size	Price	Stock
5 mg	USD 620	Ask For Quote & Lead Time
10 mg	USD 980	Ask For Quote & Lead Time
25 mg	USD 1950	Ask For Quote & Lead Time
50 mg	USD 3250	Ask For Quote & Lead Time
100 mg	USD 5200	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of IDO1/2-IN-1 hydrochloride:

Other Forms of IDO1/2-IN-1 hydrochloride:

IDO1/2-IN-1 In-stock

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IDO1 IDO2 IDO

Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

521.73

Formula

C₁₆H₁₉BrClFN₈O₄

CAS No.

2310286-60-1

SMILES

FC1=CC=C(C=C1Br)/N=C(C2=NON=C2NCCN3N=NC(COCCO)=C3)/NO.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (191.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9167 mL	9.5835 mL	19.1670 mL
5 mM	0.3833 mL	1.9167 mL	3.8334 mL
10 mM	0.1917 mL	0.9584 mL	1.9167 mL

View the Complete Stock Solution Preparation Table

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.79 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.79 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (4.79 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. He X, et al. Discovery of the First Potent IDO1/IDO2 Dual Inhibitors: A Promising Strategy for Cancer Immunotherapy. J Med Chem. 2021;64(24):17950-17968. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9167 mL	9.5835 mL	19.1670 mL	47.9175 mL
	5 mM	0.3833 mL	1.9167 mL	3.8334 mL	9.5835 mL
	10 mM	0.1917 mL	0.9584 mL	1.9167 mL	4.7918 mL
	15 mM	0.1278 mL	0.6389 mL	1.2778 mL	3.1945 mL
	20 mM	0.0958 mL	0.4792 mL	0.9584 mL	2.3959 mL
	25 mM	0.0767 mL	0.3833 mL	0.7667 mL	1.9167 mL
	30 mM	0.0639 mL	0.3195 mL	0.6389 mL	1.5973 mL
	40 mM	0.0479 mL	0.2396 mL	0.4792 mL	1.1979 mL
	50 mM	0.0383 mL	0.1917 mL	0.3833 mL	0.9584 mL
	60 mM	0.0319 mL	0.1597 mL	0.3195 mL	0.7986 mL
	80 mM	0.0240 mL	0.1198 mL	0.2396 mL	0.5990 mL
	100 mM	0.0192 mL	0.0958 mL	0.1917 mL	0.4792 mL