fMIFL
Based on 1 Customer Validation
fMIFL is a formyl tetrapeptide derived from Staphylococcus aureus, and also an agonist of mouse formyl peptide receptor 1 (mFPR1). It exhibits nanomolar activity against mFPR1 and micromolar activity against mFPR2. fMIFL is applicable to studies related to bacterial infections.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.79%
- CAS 番号: 112275-26-0
- 分子式: C27H42N4O6S
- 分子量:550.71
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
fMIFL (EC50 value; 60 min) potently induces chemotaxis in human neutrophils with an EC50 of 0.30 nM[1].
fMIFL (EC50 value; 15 min) potently induces superoxide anion production in human neutrophils with an EC50 of 1.5 nM[1].
fMIFL (EC50 value) potently induces intracellular calcium mobilization in human neutrophils with an EC50 of 0.09 nM[1].
fMIFL (1 pM-10 μM) potently activates mFPR1 in transfected RBL-2H3 cells, with an EC50 of ~10 nM for calcium mobilization[2].
fMIFL (10 nM-1 μM; 2 min-5 min) induces robust ERK1/2 phosphorylation in mFPR1-transfected RBL-2H3 cells at both 10 nM and 1 μM, with 10 nM fMIFL showing greater potency than 10 nM fMLF[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
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Cell Line:RBL-2H3 cells stably transfected with mouse formyl peptide receptor 1 (mFPR1)
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Concentration:10 nM, 1 μM (ERK1/2 phosphorylation assay)
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Incubation Time:2 min, 5 min (ERK1/2 phosphorylation assay)
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Result:Induced ERK1/2 phosphorylation to similar extents at 10 nM and 1 μM.
Showed greater potency at 10 nM than fMLF, which required 1 μM for equivalent phosphorylation.
化学情報
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CAS 番号 112275-26-0
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性状 Solid
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分子量 550.71
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分子式 C27H42N4O6S
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Color White to off-white
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配列
{N-formyl}-Met-Ile-Phe-Leu
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シーケンスの短縮
{N-formyl}-MIFL
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
H2O : 7.1 mg/mL (12.89 mM; ultrasonic and warming and adjust pH to 8 with NH4OH)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Miyazaki M, et al. Dimeric chemotactic peptides discriminate between chemotaxis and superoxide production of human neutrophils. J Biochem. 1995;117(3):489-494. [Content Brief]
[2]. Southgate EL, et al. Identification of formyl peptides from Listeria monocytogenes and Staphylococcus aureus as potent chemoattractants for mouse neutrophils. J Immunol. 2008;181(2):1429-1437. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8158 mL | 9.0792 mL | 18.1584 mL | 45.3959 mL |
| 5 mM | 0.3632 mL | 1.8158 mL | 3.6317 mL | 9.0792 mL | |
| 10 mM | 0.1816 mL | 0.9079 mL | 1.8158 mL | 4.5396 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
fMIFL 関連分類
HY-P10724 関連分類
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