MRC37
Based on 1 Customer Validation
MRC37 is a high-affinity TRIM21 ligand with a Kd of 0.4 µM. MRC37 targets the IgG Fc-binding pocket in the PRYSPRY domain of TRIM21, mimics the "HHNY" epitope, and inhibits substrate-induced aggregation and activation of TRIM21 via competitive blockade of antibody binding. MRC37 effectively blocks TRIM21-mediated antiviral immunity (such as adenovirus neutralization) and Trim-Away targeted protein degradation without cytotoxicity. MRC37 antagonizes TRIMTAC activity, and its multiple modification sites do not affect target binding, making it suitable as a degrader warhead for research on viral immunity and neurodegenerative diseases such as tauopathies.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.90%
- 分子式: C30H41N5O4
- 分子量:535.68
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
[1]|
TRIM21 PRYSPRY 0.4 μM (Kd) |
体外実験
MRC37 (0.4 μM; 48 h) binds to purified TRIM21 PRYSPRY domain with high affinity (0.4 μM) and exhibits no TRIM21-dependent cytotoxicity in wild-type RPE-1 cells at concentrations up to 20 μM for 48 h[1].
MRC37 (1 μM) monovalent ligand does not drive ternary complex formation between TRIM21 and H2B-mEGFP-Halo in RPE-1 cells, nor does it affect TRIM21 PRYSPRY binding when modified to generate TRIMTAC derivatives[1].
MRC37 (0.01-100 μM; 48 h) does not induce degradation of monomeric mEGFP-Halo, oligomeric CAV1-mEGFP-Halo, or oligomeric Cavin1-mEGFP-Halo in RPE-1 cells at concentrations up to 100 μM for 48 h[1].
MRC37 (50-100 μM; 15 h) inhibits TRIM21-mediated antiviral activity in HEK293T cells and TRIM21-dependent protein degradation in RPE-1 cells in a dose-dependent manner[1].
MRC37 (5 μM) does not degrade oligomeric Myd88 Myddosomes in stimulated cells, but can compete with TRIMTAC MRC414 to partially reverse inhibition of seeded tau aggregation in HEK293 reporter cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Solid
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分子量 535.68
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分子式 C30H41N5O4
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Color White to off-white
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SMILES
O=C(C[C@H](N)C1=C(OC)C=CC=C1)N(C2)[C@H](C(N[C@@H](CC3=CC=NC=C3)C(NC)=O)=O)C[C@H]2C4CCCCC4
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : ≥ 250 mg/mL (466.70 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8668 mL | 9.3339 mL | 18.6679 mL | 46.6697 mL |
| 5 mM | 0.3734 mL | 1.8668 mL | 3.7336 mL | 9.3339 mL | |
| 10 mM | 0.1867 mL | 0.9334 mL | 1.8668 mL | 4.6670 mL | |
| 15 mM | 0.1245 mL | 0.6223 mL | 1.2445 mL | 3.1113 mL | |
| 20 mM | 0.0933 mL | 0.4667 mL | 0.9334 mL | 2.3335 mL | |
| 25 mM | 0.0747 mL | 0.3734 mL | 0.7467 mL | 1.8668 mL | |
| 30 mM | 0.0622 mL | 0.3111 mL | 0.6223 mL | 1.5557 mL | |
| 40 mM | 0.0467 mL | 0.2333 mL | 0.4667 mL | 1.1667 mL | |
| 50 mM | 0.0373 mL | 0.1867 mL | 0.3734 mL | 0.9334 mL | |
| 60 mM | 0.0311 mL | 0.1556 mL | 0.3111 mL | 0.7778 mL | |
| 80 mM | 0.0233 mL | 0.1167 mL | 0.2333 mL | 0.5834 mL | |
| 100 mM | 0.0187 mL | 0.0933 mL | 0.1867 mL | 0.4667 mL |
MRC37 関連分類
HY-180548 関連分類
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