p53 CBS
p53 CBS (p53 Consensus binding sequence) is a biological active peptide. (p53 consensus DNA binding site)
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- CAS 番号: 721963-21-9
- 分子式: C144H184N42O27S2
- 分子量:2999.40
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
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Help & FAQs
生物活性
製品説明
化学情報
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CAS 番号 721963-21-9
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分子量 2999.40
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分子式 C144H184N42O27S2
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別名
p53 Consensus binding sequence
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配列
Arg-Arg-Arg-Cys-Trp-Trp-Gly-Tyr-Tyr-Tyr-Arg-Arg-Arg-Cys-Trp-Trp-Gly-Tyr-Tyr-Tyr
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シーケンスの短縮
RRRCWWGYYYRRRCWWGYYY
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Protocol for Electrophoretic Mobility Shift Assay (EMSA)
Electrophoretic mobility shift assay detects protein-nucleic acid binding by incubating a labeled DNA or RNA probe with purified protein or cell extract, then separating free probe from slower-migrating protein-probe complexes on a native gel. For cancer cells, primary neurons, mouse tumor samples, intestinal organoids, inflammatory macrophages, or drug-treated samples, EMSA can measure transcription-factor DNA binding or RNA-binding protein activity in extracts, but it does not directly measure transcription, protein expression, or chromatin occupancy in intact cells. Specificity is judged by competition with unlabeled wild-type probe, failure of mutated or unrelated competitors to compete, and antibody supershift or disruption when the binding protein identity must be confirmed.
純度とドキュメンテーション
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
p53 CBS 関連分類
HY-P5343 関連分類
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