ZL-1211
ZL-1211 is an anti-CLDN18.2 monoclonal antibody. ZL-1211 exhibits enhanced binding affinity to CD16A. ZL-1211 induces ADCC-mediated cell lysis and triggers the production of IFNγ, TNFα and IL6. ZL-1211 promotes antibody-dependent cellular cytotoxicity. ZL-1211 exerts anti-tumor activity in a mouse xenograft model of gastric cancer. ZL-1211 can be used for the research of gastric cancer.
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
[1]|
IL-6 |
体外実験
ZL-1211 (1 h) specifically binds to CLDN18.2 on the surface of CHO cells and NUGC4 cells with overexpressed CLDN18.2[1].
ZL-1211 (serial dilution concentrations; 4-5 h) potently induces ADCC against CLDN18.2-overexpressing NUGC4-hCLDN18.2 cells via PBMC effector cells[1].
ZL-1211 (at serially diluted concentrations) potently induces ADCC effects against MIA-PaCa2-hCLDN18.2 cell clones with high, medium, and low CLDN18.2 expression via purified NK cells[1].
ZL-1211 (0.1 μg/mL; 20 min) potently induces complement-dependent cytotoxicity (CDC) against MIA-PaCa2-hCLDN18.2 cell clones with high and moderate CLDN18.2 expression via serum complement[1].
ZL-1211 (at serially diluted concentrations) potently induces antibody-dependent cell-mediated cytotoxicity (ADCC) against gastric cancer cell lines with high (SNU601), moderate (SNU620), and low (KATOIII) endogenous CLDN18.2 expression via purified NK cells at E:T ratios of 1:1 and 3:1. Its efficacy is significantly superior to that of clinical benchmark analogs, and it shows no activity against CLDN18.2-negative SNU5 cells[1].
ZL-1211 (0.01 μg/mL; 3 days) activates NK cells co-cultured with SNU601 gastric cancer cells, as evidenced by a significant increase in intracellular perforin levels compared with the control hIgG1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CLDN18.2-overexpressing NUGC4-hCLDN18.2, peripheral blood mononuclear cell (PBMC) effector cells
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Concentration:serially diluted concentrations (cytotoxicity assessment)
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Incubation Time:4-5 hours (incubation at 37°C)
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Result:Induced robust ADCC-mediated tumor cell death, with greater potency than ZL-1211 wild-type Fc and the benchmark analog.
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Cell Line:CLDN18.2-high, -medium, -low MIA-PaCa2-hCLDN18.2
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Concentration:0.1 μg/mL (CDC induction in high/medium clones)
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Incubation Time:20 minutes (pre-incubation at room temperature); 30 minutes (incubation at 37°C with complement)
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Result:Induced CDC-mediated tumor cell death in CLDN18.2-high and -medium clones at 0.1 μg/mL, while the benchmark analog required a much higher concentration to induce CDC.
体内実験
ZL-1211 (10 mg/kg; i.p.; once weekly) exhibits significant antitumor efficacy against CLDN18.2-low-expressing KATOIII gastric cancer tumors in Balb/c nude mice[1].
ZL-1211 (5 mg/kg; i.p.; once weekly) exerts NK cell-dependent antitumor efficacy against CLDN18.2-highly expressing SNU601 gastric cancer in Balb/c nude mice[1].
ZL-1211 (50 mg/kg; i.p.; three times per week) exerts dose-dependent anti-tumor efficacy against CLDN18.2-expressing gastric cancer PDX models in Balb/c nude mice, while enhancing NK cell infiltration into responsive tumors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Balb/c nude mice (7-9 weeks old, female)[1]
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Dosage:0.1 mg/kg; 1 mg/kg; 10 mg/kg
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Administration:i.p.; once weekly
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Result:Suppressed SNU620 tumor growth at all tested doses.
Achieved significant tumor inhibition at 0.1 mg/kg, 1 mg/kg, and 10 mg/kg doses at day 42, with similar inhibition to benchmark antibody at 10 mg/kg despite having ~twice lower serum exposure than the benchmark.
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Animal Model:Balb/c nude mice (7-9 weeks old, female)[1]
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Dosage:10 mg/kg
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Administration:i.p.; once weekly
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Result:Significantly suppressed KATOIII tumor growth, with smaller mean tumor volumes compared to the benchmark antibody at day 40.
Did not inhibit growth of CLDN18.2-negative SNU5 tumors.
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Animal Model:Balb/c nude mice; NOD.SCID mice; NCG mice[1]
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Dosage:5 mg/kg
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Administration:i.p.; once weekly
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Result:Significantly suppressed SNU601 tumor growth in Balb/c nude mice, with mean tumor volumes ~50% lower than control at day 60.
Showed minimal to no tumor growth inhibition in NOD.SCID mice and NCG mice, correlating with tumor NK cell levels.
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Animal Model:Balb/c nude mice[1]
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Dosage:50 mg/kg
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Administration:i.p.; three times weekly
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Result:Induced tumor growth inhibition (TGI) in 3 of 7 PDX models: GA0006 (TGI = 56.6%), GA6831 (TGI = 56.0%), and GA2419 (TGI = 40.3%).
No significant TGI was observed in CLDN18.2-negative PDX models.
Increased NK cell density significantly in treated tumors compared to control in the responder PDX group.
遺伝子ID
アクセッション番号
Target
CLDN18.2
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
アプリケーション
ELISA, FACS, Functional assay
化学情報
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
ZL-1211 関連分類
HY-P992487 関連分類
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