Acifluorfen
Based on 1 Customer Validation
Acifluorfen, a protoporphyrinogen oxidase (PROTOX) inhibitor herbicide, promotes the accumulation of protoporphyrin IX (PPIX), and induces tumors in the rodent liver. Acifluorfen causes strong photooxidative destruction of pigments and lipids in sensitive plant species.
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- Purity : 98.95%
- CAS No.: 50594-66-6
- 화학식: C14H7ClF3NO5
- 분자량:361.66
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
The effect of Acifluorfen on glutathione and ascorbate levels in cucumber (Cucumis sativus L.) cotyledon discs is investigated to assess the relationship between herbicide activity and endogenous antioxidants. Acifluorfen decreases the levels of glutathione and ascorbate over 50% in discs exposed to less than 1.5 hours of white light (450 microeinsteins per square meter per second). Acifluorfen also causes the rapid depletion of ascorbate in far-red light grown plants which are photosynthetically incompetent[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Induced Disease Models
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Acifluorfen can be used in animal modeling to create liver injury and cardiac disease models. Dietary treatment with 2500 ppm Acifluorfen for up to 13 weeks increases the expression of Cyp2b10 in the liver of wild-type mice but does not increase liver expression in constitutive androstane receptor gene knockout (CARKO) mice. Under the microscope, Acifluorfen induces cytotoxic changes, including hepatocellular necrosis and inflammation, and causes regenerative changes, accompanied by a long-term increase in the number of proliferating cell nuclear antigen-positive liver cells in WT mice[1].
Administration: 2500 μg/mL• mixed in the basal diet• daily for 26 weeks[1]
Molecular changes: Acifluorfen induced hepatocellular hypertrophy, then hepatocellular hypertrophy and spread from the centrilobular to the midzonal or panlobular area with time, showing immunoreactivity for CYP2B, CYP2A and CYP4A[1].
Metabolic change: Acifluorfen induced mitosis and karyocytomegaly (increase in nuclear size) in hepatocytes more frequently[1].
Administration: 0, 20, 40 mg/L• exposure to a working solution• exposed for 96 h[2]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 50594-66-6
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Appearance Solid
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분자량 361.66
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화학식 C14H7ClF3NO5
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Color White to off-white
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SMILES
O=C(O)C1=CC(OC2=CC=C(C(F)(F)F)C=C2Cl)=CC=C1[N+]([O-])=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 25 mg/mL (69.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.91 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (281 KB)
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SDS (619 KB)
- English - EN (619 KB)
- Français - FR (619 KB)
- Deutsch - DE (619 KB)
- Norwegian - NO (619 KB)
- Español - ES (619 KB)
- Swedish - SV (619 KB)
- Italian - IT (619 KB)
- Korean - KR (619 KB)
- Portuguese - PT (619 KB)
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Handling Instructions (2659 KB)
References
[1]. Kuwata K, et al. Involvement of Mouse Constitutive Androstane Receptor in Acifluorfen-Induced Liver Injury and Subsequent Tumor Development. Toxicol Sci. 2016;151(2):271-285. [Content Brief]
[2]. Hong T, et al. Exposure to acifluorfen induces developmental toxicity in the early life stage of zebrafish. Comp Biochem Physiol C Toxicol Pharmacol. 2024 Jul;281:109909. [Content Brief]
[3]. Kenyon WH, et al. Effects of Acifluorfen on Endogenous Antioxidants and Protective Enzymes in Cucumber (Cucumis sativus L.) Cotyledons. Plant Physiol. 1985;79(3):862-866. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7650 mL | 13.8251 mL | 27.6503 mL | 69.1257 mL |
| 5 mM | 0.5530 mL | 2.7650 mL | 5.5301 mL | 13.8251 mL | |
| 10 mM | 0.2765 mL | 1.3825 mL | 2.7650 mL | 6.9126 mL | |
| 15 mM | 0.1843 mL | 0.9217 mL | 1.8434 mL | 4.6084 mL | |
| 20 mM | 0.1383 mL | 0.6913 mL | 1.3825 mL | 3.4563 mL | |
| 25 mM | 0.1106 mL | 0.5530 mL | 1.1060 mL | 2.7650 mL | |
| 30 mM | 0.0922 mL | 0.4608 mL | 0.9217 mL | 2.3042 mL | |
| 40 mM | 0.0691 mL | 0.3456 mL | 0.6913 mL | 1.7281 mL | |
| 50 mM | 0.0553 mL | 0.2765 mL | 0.5530 mL | 1.3825 mL | |
| 60 mM | 0.0461 mL | 0.2304 mL | 0.4608 mL | 1.1521 mL |
Acifluorfen Related Classifications
HY-128075 Related Classifications
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