Mito-PN

In the fluorescence spectra, the solution of free probe Mito-PN (5 μM) displays a negligible fluorescence emission in the aqueous solution containing phosphate buffered solution (PBS, 20 mM, pH = 7.4). The non-fluorescence property of probe Mito-PN might be ascribed to the inherent structure of a spirolactone. As expected, the considerable fluorescence enhancement (about 40-fold) at 630 nm was observed upon addition of ONOO^-. Surprisingly, a fast response of probe Mito-PN for detecting ONOO^- (<5 s) was achieved, and this feature is advantageous than the reported conjugation of rhodamine and phenylhydrazine (the complete reaction needs about 1 h). The fast response enables probe Mito-PN convenient to track the highly reactive and short-lived ONOO^- in the biological systems. Meanwhile, the absorption spectral properties of probe Mito-PN were also investigated. Probe Mito-PN exhibited an obvious increase at about 570 nm upon addition of ONOO^-[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.