L-Methionine-DL-sulfoximine
Based on 6 publication(s) in Google Scholar
L-Methionine-DL-sulfoximine (MSX; MSO), a highly specific and irreversible inhibitor of Glutamine synthetase (GS), is also a potent convulsant which metabolically and morphologically primarily affects astroglia. L-Methionine-DL-sulfoximine has been employed to inhibit the Gln-dependent ammonia-stimulated neuronal toxicity in vitro, potentiating Gln deficit-dependent depression. L-Methionine-DL-sulfoximine tremendously increases the rate of release of fixed nitrogen in cyanobacteria. L-Methionine-DL-sulfoximine is a promising candidate for research in biofertilizers and convulsive seizures (CS).
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 15985-39-4
- Formula: C5H12N2O3S
- Molecular Weight:180.23
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Methionine-DL-sulfoximine
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Customer Validation & Images
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Histological Imaging/Staining
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Bio/Physico-chemical Assay
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Histological Imaging/Staining
Biological Activity
L-Methionine-DL-sulfoximine (75 mg/kg, i.p.) reduces D-Asp release evoked by K+-induced depolarization and unchanges Glu uptake in the hippocampus and enthorhinal cortex of temporal lobe epilepsy (TLE) model rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male rats (24 days old, Sprague Dawley)[1]
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Dosage:75 mg/kg
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Administration:210 min, i.p., measurements were taken at MSO “0”, after 15 min (referred to as MSO “15”, Pilo “15” and MSO + Pilo “15”) and after 60 min (MSO “60”, Pilo “60” and MSO + Pilo “60”, respectively
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Result:Did not affect the hippocampal GS activity and the expression of GS protein for the first 150 min, but significantly decreased the activity during 165-210 min after MSO administration in rats. Additionally, MSO reduced the intensity of CS caused by Pilo administration.
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Animal Model:Hippocampus and enthorhinal cortex of TLE model rats[4]
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Dosage:75 mg/kg
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Administration:i.p., Administration 150 minutes before decapitation
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Result:Markedly reduced D-Asp release evoked by K+-induced depolarization and slightly decreased [3H]D-Asp uptake at higher concentrations of [3H]D-Asp in TLE model rats.
Chemical Information
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CAS No. 15985-39-4
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Appearance Solid
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Molecular Weight 180.23
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Formula C5H12N2O3S
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Color White to off-white
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SMILES
O=C(O)[C@@H](N)CCS(=N)(C)=O
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Synonyms
MSX; MSO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell
Gut-to-brain signaling restricts dietary protein intake during recovery from catabolic states. [Abstract]2025 Dec 24;188(26):7481-7494.e16. PMID: 41192423 -
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J Agric Food Chem
Butyrate Alleviates High-Fat-Induced Metabolic Disorders Partially through Increasing Systematic Glutamine. [Abstract]2024 Jan 10;72(1):449-460. PMID: 38109504
L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]
MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). H&E staining of iWAT and eWAT sections.
L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]
MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). Weight of adipose tissues (iWAT, eWAT, and BAT) in mice (n = 7).
L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]
MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). Ratio of fat mass to body weight (n = 7).
L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]
MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). The content of TG in serum.
L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]
MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). H&E staining images of liver sections. Scale bar = 200 μm.
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Biomedicines
Targeting the Glutamine Transporter SLC1A5 Enhances Sensitivity of Acute Myeloid Leukemia to MLN4924. [Abstract]2026 Mar 14;14(3):667. PMID: 41898313 -
Pathol Res Pract
Glutamine metabolites promote the progression of cervical cancer by inducing M2 macrophage polarization. [Abstract]2026 Feb:278:156348. PMID: 41478261 -
Animals (Basel)
Effects of Glutamine or Glucose Deprivation on Inflammation and Tight Junction Disruption in Yak Rumen Epithelial Cells. [Abstract]2024 Nov 12;14(22):3232. PMID: 39595285
Solvent & Solubility
H2O : 50 mg/mL (277.42 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (554.85 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Pawlik MJ, et al. Pretreatment with a glutamine synthetase inhibitor MSO delays the onset of initial seizures induced by pilocarpine in juvenile rats[J]. Brain Res. 2021 Feb 15;1753:147253. [Content Brief]
[3]. Albrecht J, Norenberg MD. L-methionine-DL-sulfoximine induces massive efflux of glutamine from cortical astrocytes in primary culture. Eur J Pharmacol. 1990;182(3):587-589. [Content Brief]
[4]. Pawlik MJ, et al. Inhibition of Glutamate Release, but Not of Glutamine Recycling to Glutamate, Is Involved in Delaying the Onset of Initial Lithium-Pilocarpine-Induced Seizures in Young Rats by a Non-Convulsive MSO Dose[J]. Int J Mol Sci. 2021 Oct 15;22(20):11127. [Content Brief]
[5]. Premakumar R, Sorger GJ, Gooden D. Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b. J Bacteriol. 1980;143(1):411-415. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 5.5485 mL | 27.7423 mL | 55.4847 mL | 138.7116 mL |
| 5 mM | 1.1097 mL | 5.5485 mL | 11.0969 mL | 27.7423 mL | |
| 10 mM | 0.5548 mL | 2.7742 mL | 5.5485 mL | 13.8712 mL | |
| 15 mM | 0.3699 mL | 1.8495 mL | 3.6990 mL | 9.2474 mL | |
| 20 mM | 0.2774 mL | 1.3871 mL | 2.7742 mL | 6.9356 mL | |
| 25 mM | 0.2219 mL | 1.1097 mL | 2.2194 mL | 5.5485 mL | |
| 30 mM | 0.1849 mL | 0.9247 mL | 1.8495 mL | 4.6237 mL | |
| 40 mM | 0.1387 mL | 0.6936 mL | 1.3871 mL | 3.4678 mL | |
| 50 mM | 0.1110 mL | 0.5548 mL | 1.1097 mL | 2.7742 mL | |
| 60 mM | 0.0925 mL | 0.4624 mL | 0.9247 mL | 2.3119 mL | |
| 80 mM | 0.0694 mL | 0.3468 mL | 0.6936 mL | 1.7339 mL | |
| 100 mM | 0.0555 mL | 0.2774 mL | 0.5548 mL | 1.3871 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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