L-Methionine-DL-sulfoximine (Synonyms: MSX; MSO)

Name: L-Methionine-DL-sulfoximine
Brand: MedChemExpress
SKU: HY-B1692
Rating: 5.0 (6 reviews)

Cat. No.: HY-B1692 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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L-Methionine-DL-sulfoximine (MSX; MSO), a highly specific and irreversible inhibitor of Glutamine synthetase (GS), is also a potent convulsant which metabolically and morphologically primarily affects astroglia. L-Methionine-DL-sulfoximine has been employed to inhibit the Gln-dependent ammonia-stimulated neuronal toxicity in vitro, potentiating Gln deficit-dependent depression. L-Methionine-DL-sulfoximine tremendously increases the rate of release of fixed nitrogen in cyanobacteria. L-Methionine-DL-sulfoximine is a promising candidate for research in biofertilizers and convulsive seizures (CS).

For research use only. We do not sell to patients.

CAS No. : 15985-39-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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Based on 6 publication(s) in Google Scholar

6 Publications Citing Use of MCE L-Methionine-DL-sulfoximine

Histological Imaging/Staining

In Vivo Efficacy Study

Bio/Physico-chemical Assay

: L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]; MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). H&E staining of iWAT and eWAT sections.

: L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]; MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). Weight of adipose tissues (iWAT, eWAT, and BAT) in mice (n = 7).

: L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]; MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). Ratio of fat mass to body weight (n = 7).

: L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]; MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). The content of TG in serum.

: L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460. [Abstract]; MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). H&E staining images of liver sections. Scale bar = 200 μm.

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Description

In Vivo

L-Methionine-DL-sulfoximine (75 mg/kg, i.p., 210 min) exerts no affect on the hippocampal Gutamine synthetase (GS) activity and the expression of GS protein for the first 150 min, but significantly decreases the activity of GS during 165-210 min in rats. Additionally, L-Methionine-DL-sulfoximine also reduces the intensity of convulsive seizures (CS) caused by Pilocarpine (Pilo) (HY-B0726A) administration and seizure in rats^[1].
L-Methionine-DL-sulfoximine (75 mg/kg, i.p.) reduces D-Asp release evoked by K⁺-induced depolarization and unchanges Glu uptake in the hippocampus and enthorhinal cortex of temporal lobe epilepsy (TLE) model rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male rats (24 days old, Sprague Dawley)^[1]
Dosage:	75 mg/kg
Administration:	210 min, i.p., measurements were taken at MSO “0”, after 15 min (referred to as MSO “15”, Pilo “15” and MSO + Pilo “15”) and after 60 min (MSO “60”, Pilo “60” and MSO + Pilo “60”, respectively
Result:	Did not affect the hippocampal GS activity and the expression of GS protein for the first 150 min, but significantly decreased the activity during 165-210 min after MSO administration in rats. Additionally, MSO reduced the intensity of CS caused by Pilo administration.

Animal Model:	Hippocampus and enthorhinal cortex of TLE model rats^[4]
Dosage:	75 mg/kg
Administration:	i.p., Administration 150 minutes before decapitation
Result:	Markedly reduced D-Asp release evoked by K⁺-induced depolarization and slightly decreased [³H]D-Asp uptake at higher concentrations of [³H]D-Asp in TLE model rats.

Molecular Weight

180.23

Formula

C₅H₁₂N₂O₃S

CAS No.

15985-39-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@@H](N)CCS(=N)(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (277.42 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.5485 mL	27.7423 mL	55.4847 mL
5 mM	1.1097 mL	5.5485 mL	11.0969 mL
10 mM	0.5548 mL	2.7742 mL	5.5485 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (554.85 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (248 KB) HNMR (265 KB) MS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Pawlik MJ, et al. Pretreatment with a glutamine synthetase inhibitor MSO delays the onset of initial seizures induced by pilocarpine in juvenile rats[J]. Brain Res. 2021 Feb 15;1753:147253. [Content Brief]

[2]. Prakash, O., et al. Isolation and Characterization of Ammonia Secreting Cyanobacterium Nostoc sp. NDUPC007 from Agriculture Fields of Varanasi[J]. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 2022 January 10; 92, 89–93.

[3]. Albrecht J, Norenberg MD. L-methionine-DL-sulfoximine induces massive efflux of glutamine from cortical astrocytes in primary culture. Eur J Pharmacol. 1990;182(3):587-589. [Content Brief]

[4]. Pawlik MJ, et al. Inhibition of Glutamate Release, but Not of Glutamine Recycling to Glutamate, Is Involved in Delaying the Onset of Initial Lithium-Pilocarpine-Induced Seizures in Young Rats by a Non-Convulsive MSO Dose[J]. Int J Mol Sci. 2021 Oct 15;22(20):11127. [Content Brief]

[5]. Premakumar R, Sorger GJ, Gooden D. Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b. J Bacteriol. 1980;143(1):411-415. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.5485 mL	27.7423 mL	55.4847 mL	138.7116 mL
	5 mM	1.1097 mL	5.5485 mL	11.0969 mL	27.7423 mL
	10 mM	0.5548 mL	2.7742 mL	5.5485 mL	13.8712 mL
	15 mM	0.3699 mL	1.8495 mL	3.6990 mL	9.2474 mL
	20 mM	0.2774 mL	1.3871 mL	2.7742 mL	6.9356 mL
	25 mM	0.2219 mL	1.1097 mL	2.2194 mL	5.5485 mL
	30 mM	0.1849 mL	0.9247 mL	1.8495 mL	4.6237 mL
	40 mM	0.1387 mL	0.6936 mL	1.3871 mL	3.4678 mL
	50 mM	0.1110 mL	0.5548 mL	1.1097 mL	2.7742 mL
	60 mM	0.0925 mL	0.4624 mL	0.9247 mL	2.3119 mL
	80 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7339 mL
	100 mM	0.0555 mL	0.2774 mL	0.5548 mL	1.3871 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-Methionine-DL-sulfoximine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Methionine-DL-sulfoximine15985-39-4MSX MSOGlutaminaseglutamine synthetaseconvulsantastrogliadepressionfixed nitrogenbiofertilizersconvulsive seizuresInhibitorinhibitorinhibit

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L-Methionine-DL-sulfoximine (Synonyms: MSX; MSO)

Customer Review

L-Methionine-DL-sulfoximine Related Antibodies

6 Publications Citing Use of MCE L-Methionine-DL-sulfoximine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

L-Methionine-DL-sulfoximine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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