Magnoloside B
Based on 1 Customer Validation
Magnoloside B is a phenylethanoid glycoside found in the bark of Magnolia officinalis. Magnoloside B exhibits DPPH radical scavenging activity. Magnoloside B can be used in research related to liver cancer and gastric cancer.
For research use only. We do not sell to patients.
- Purity : 98.40%
- CAS No.: 116872-05-0
- Formula: C35H46O20
- Molecular Weight:786.73
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MGC-803 | IC50 |
21.81 μM
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Cytotoxicity against human stomach cancer MGC-803 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
Cytotoxicity against human stomach cancer MGC-803 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
|
27086163 |
| HepG2 | IC50 |
28.05 μM
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Cytotoxicity against human liver cancer HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
Cytotoxicity against human liver cancer HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
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27086163 |
| Vero | IC50 |
60.21 μM
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Cytotoxicity against normal kidney Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
Cytotoxicity against normal kidney Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
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27086163 |
In Vitro
Magnoloside B (0.005-0.5 mg/mL; 30 min) exhibits potent DPPH radical scavenging activity[1].
Magnoloside B (compound 25) (1 mM; 10 min pre-incubation, 20 min incubation) moderately inhibits α-glucosidase from Saccharomyces cerevisiae with an IC50 of 0.69 mM[2].
Magnoloside B (100 μM; 72 h) is moderately cytotoxic against MGC-803 (IC50 21.81 μM) and HepG2 (IC50 28.05 μM) cell lines but shows no activity against PC3, MCF-7, PC12, or A549 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MGC-803; HepG2; PC3; MCF-7; PC12; A549; Vero
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Concentration:100 μM (initial); 21.81 μM (MGC-803); 28.05 μM (HepG2); 60.21 μM (Vero)
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Incubation Time:72 h
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Result:Showed moderate cytotoxic activity against MGC-803 with an IC50 of 21.81 μM and against HepG2 with an IC50 of 28.05 μM.
Showed some toxicity against normal Vero cells with an IC50 of 60.21 μM.
Showed no significant cytotoxic activity against PC3, MCF-7, PC12, or A549 cell lines (IC50 > 100 μM).
Chemical Information
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CAS No. 116872-05-0
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Appearance Solid
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Molecular Weight 786.73
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Formula C35H46O20
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Color Light yellow to yellow
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SMILES
O[C@H]([C@@H](CO[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)O2)[C@@H](OC(/C=C/C3=CC=C(O)C(O)=C3)=O)[C@@H](O[C@H]4[C@@H]([C@@H]([C@H]([C@H](C)O4)O)O)O)[C@@H]2OCCC5=CC=C(O)C(O)=C5
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (127.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
Purity & Documentation
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Data Sheet (297 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang M, et al. Rapid purification of antioxidants from Magnolia officinalis by semi-prep-HPLC with a two-step separation strategy guided by on-line HPLC-radical scavenging detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Nov 15;1100-1101:140-147. [Content Brief]
[2]. Xue Z, et al. Phenylethanoid glycosides and phenolic glycosides from stem bark of Magnolia officinalis. Phytochemistry. 2016 Jul;127:50-62. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2711 mL | 6.3554 mL | 12.7108 mL | 31.7771 mL |
| 5 mM | 0.2542 mL | 1.2711 mL | 2.5422 mL | 6.3554 mL | |
| 10 mM | 0.1271 mL | 0.6355 mL | 1.2711 mL | 3.1777 mL | |
| 15 mM | 0.0847 mL | 0.4237 mL | 0.8474 mL | 2.1185 mL | |
| 20 mM | 0.0636 mL | 0.3178 mL | 0.6355 mL | 1.5889 mL | |
| 25 mM | 0.0508 mL | 0.2542 mL | 0.5084 mL | 1.2711 mL | |
| 30 mM | 0.0424 mL | 0.2118 mL | 0.4237 mL | 1.0592 mL | |
| 40 mM | 0.0318 mL | 0.1589 mL | 0.3178 mL | 0.7944 mL | |
| 50 mM | 0.0254 mL | 0.1271 mL | 0.2542 mL | 0.6355 mL | |
| 60 mM | 0.0212 mL | 0.1059 mL | 0.2118 mL | 0.5296 mL | |
| 80 mM | 0.0159 mL | 0.0794 mL | 0.1589 mL | 0.3972 mL | |
| 100 mM | 0.0127 mL | 0.0636 mL | 0.1271 mL | 0.3178 mL |
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