Myxopyronin B
Myxopyronin B is an α-pyrone natural product derived from Myxococcus fulvus and acts as an inhibitor of the switch region of bacterial RNA polymerase (RNAP) (IC50 = 24 μM). Myxopyronin B binds to the switch region of RNA polymerase, locking the polymerase clamp into a closed conformation and thereby inhibiting transcription initiation. Myxopyronin B exhibits antibacterial activity against Staphylococcus aureus and Clostridioides difficile, inhibits the early synthesis of toxins A and B, and reduces the abundance of proteins in the branched-chain amino acid fermentation pathway. Myxopyronin B is useful for research related to bacterial infections.
For research use only. We do not sell to patients.
- CAS No.: 88192-99-8
- Formula: C24H33NO6
- Molecular Weight:431.52
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
RNA Polymerase 24 μM (IC50) |
In Vitro
Myxopyronin B (MyxB) inhibits transcription by the Escherichia coli RNAP holoenzyme with an IC50 of 24 μM[1].
Myxopyronin B (500 ng/mL; 90 min) induces a proteomic stress signature in C. difficile 630 that shares features with Fidaxomicin (HY-17580) and Rifaximin (HY-13234), including increased abundance of proteins encoded by the CD630_08470-CD630_08500 operon and a trend toward increased ABC efflux systems[2].
Myxopyronin B (0-128 μg/mL) exhibits antibacterial activity against multiple Clostridioides difficile strains, including the fidaxomicin-resistant Goe-91 strain, with MIC values of 8 μg/mL for both strain 630 and Goe-91[1].
Myxopyronin B inhibits the growth of Staphylococcus aureus; upon the addition of human serum albumin, its antibacterial activity decreases by ≥128-fold, with a protein binding rate of 99.5 %[2].
Myxopyronin B (4-8 × MIC) selects for drug-resistant Staphylococcus aureus mutants that carry single amino acid substitutions in the RpoB or RpoC subunits that form the MyxB binding site, and these mutants can exhibit high-level resistance[2].
Myxopyronin B (500 ng/mL) inhibits the synthesis of toxins A and B in C. difficile 630 under in vitro conditions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Staphylococcus aureus strains ATCC 29213, ATCC 12600, and MW2
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Concentration:4 × MIC and 8 × MIC
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Incubation Time:48 h
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Result:At 4 × MIC, the average frequency of resistance was similar to rifampin for three strains of S. aureus.
At 4 × MIC, frequencies were 4.8 × 10-8 for ATCC 29213, 8.0 × 10-8 for ATCC 12600, and 1.3 × 10-7 for MW2.
At 8 × MIC, frequencies were 2.3 × 10-8 for ATCC 29213, 6.4 × 10-8 for ATCC 12600, and 1.1 × 10-7 for MW2.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 88192-99-8
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Molecular Weight 431.52
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Formula C24H33NO6
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SMILES
CCCC/C(C)=C/C=C(C)/C(C1=C(C=C(OC1=O)[C@H](C)CC/C=C/NC(OC)=O)O)=O
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Structure Classification
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Initial Source
Myxococcus fulvus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Myxopyronin B Related Classifications
HY-N21879 Related Classifications
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