N-Decanoyl cyclopentylamide
N-Decanoyl cyclopentylamide (C10-CPA) is a potent inhibitor of quorum sensing in Pseudomonas aeruginosa. N-Decanoyl cyclopentylamide interferes with expression of Pseudomonas aeruginosa virulence factors regulated by the las and rhl quorum-sensing systems. N-Decanoyl cyclopentylamide inhibits production of elastase, pyocyanin, and rhamnolipid and biofilm formation.
For research use only. We do not sell to patients.
- CAS No.: 540796-75-6
- Formula: C15H29NO
- Molecular Weight:239.40
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
N-Decanoyl cyclopentylamide (C10-CPA) inhibits quorum sensing in Pseudomonas aeruginosa, and its concentrations required for half-maximal inhibition for lasB-lacZ and rhlA-lacZ expression are 80 μM and 90 μM, respectively[1].
N-Decanoyl cyclopentylamide also inhibits production of virulence factors, including elastase, pyocyanin, and rhamnolipid, and biofilm formation without affecting growth of Pseudomonas aeruginosa PAO1[1].
N-Decanoyl cyclopentylamide interferes with the las and rhl quorum-sensing systems via inhibiting interaction between their response regulators (LasR and RhlR) and autoinducers[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 540796-75-6
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Molecular Weight 239.40
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Formula C15H29NO
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SMILES
O=C(CCCCCCCCC)NC1CCCC1
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Synonyms
C10-CPA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Fungal Biofilm Culture
Fungal biofilm culture is an in vitro method for growing surface-attached fungal communities, most commonly Candida albicans, on abiotic substrates such as polystyrene wells, silicone elastomer, or polymethylmethacrylate; the assay models adhesion, proliferation, filamentation, extracellular-matrix-associated maturation, and dispersion. Biofilm output can be read by optical density at 600 nm for adherent biomass, XTT reduction for metabolic activity, CFU recovery for viable attached or dispersed cells, and microscopy for architecture.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
N-Decanoyl cyclopentylamide Related Classifications
HY-158193 Related Classifications
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