N-Octanoyl-L-homoserine lactone
Based on 1 Customer Validation
N-Octanoyl-L-homoserine lactone (C8-HSL) is an autoinducer. It mainly serves as a key inter- and intra-species communication medium or "signal molecule" in Gram-negative bacteria. N-Octanoyl-L-homoserine lactone microparticles exhibit adjuvant potential when used in combination with various particulate vaccines.
For research use only. We do not sell to patients.
- Purity : 99.40%
- CAS No.: 147852-84-4
- Formula: C12H21NO3
- Molecular Weight:227.30
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-133685
N-Hexanoyl-L-homoserine lactone -
HY-114816
N-Butanoyl-L-homoserine lactone -
HY-W008806
N-(3-Oxohexanoyl)-L-homoserine lactone -
HY-118697
N-dodecanoyl-L-Homoserine lactone -
HY-114544A
N-3-oxo-dodecanoyl-L-homoserine lactone -
HY-136409
N-Decanoyl-L-homoserine lactone -
HY-114816R
N-Butanoyl-L-homoserine lactone (Standard) -
HY-133685R
N-Hexanoyl-L-homoserine lactone (Standard) -
HY-124237AR
N-Octanoyl-L-homoserine lactone (Standard) -
HY-108700
N-3-Oxo-octanoyl-L-homoserine lactone -
HY-P1934A
Cyclo(L-Phe-L-Pro) -
HY-14538
Haloperidol
Biological Activity
Description
In Vitro
N-Octanoyl-L-homoserine lactone microspheres (20-200 µg/mL; 24 h) show no cytotoxicity against dendritic 2.4 cells[1].
N-Octanoyl-L-homoserine lactone microspheres (20-500 µg/mL; 24 h) induce nitric oxide release from dendritic 2.4 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 147852-84-4
-
Appearance Solid
-
Molecular Weight 227.30
-
Formula C12H21NO3
-
Color White to off-white
-
SMILES
CCCCCCCC(N[C@@H]1C(OCC1)=O)=O
-
Synonyms
C8-HSL
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (439.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (11.00 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (11.00 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (269 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3995 mL | 21.9974 mL | 43.9947 mL | 109.9868 mL |
| 5 mM | 0.8799 mL | 4.3995 mL | 8.7989 mL | 21.9974 mL | |
| 10 mM | 0.4399 mL | 2.1997 mL | 4.3995 mL | 10.9987 mL | |
| 15 mM | 0.2933 mL | 1.4665 mL | 2.9330 mL | 7.3325 mL | |
| 20 mM | 0.2200 mL | 1.0999 mL | 2.1997 mL | 5.4993 mL | |
| 25 mM | 0.1760 mL | 0.8799 mL | 1.7598 mL | 4.3995 mL | |
| 30 mM | 0.1466 mL | 0.7332 mL | 1.4665 mL | 3.6662 mL | |
| 40 mM | 0.1100 mL | 0.5499 mL | 1.0999 mL | 2.7497 mL | |
| 50 mM | 0.0880 mL | 0.4399 mL | 0.8799 mL | 2.1997 mL | |
| 60 mM | 0.0733 mL | 0.3666 mL | 0.7332 mL | 1.8331 mL | |
| 80 mM | 0.0550 mL | 0.2750 mL | 0.5499 mL | 1.3748 mL | |
| 100 mM | 0.0440 mL | 0.2200 mL | 0.4399 mL | 1.0999 mL |
N-Octanoyl-L-homoserine lactone Related Classifications
HY-124237A Related Classifications
Powered by Bioz