N-Butanoyl-L-homoserine lactone
Based on 3 publication(s) in Google Scholar
N-Butanoyl-L-homoserine lactone (C4-HSL) is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). N-Butanoyl-L-homoserine lactone has antibacterial activity and is used in antibacterial biofilm. N-Butanoyl-L-homoserine lactone aptamers blocks qurom sensing and inhibits biofilm formation in Pseudomonas aeruginosa.
For research use only. We do not sell to patients.
- Purity : 99.95%
- CAS No.: 67605-85-0
- Formula: C8H13NO3
- Molecular Weight:171.20
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-133685
N-Hexanoyl-L-homoserine lactone -
HY-124237A
N-Octanoyl-L-homoserine lactone -
HY-136409
N-Decanoyl-L-homoserine lactone -
HY-114544A
N-3-oxo-dodecanoyl-L-homoserine lactone -
HY-118697
N-dodecanoyl-L-Homoserine lactone -
HY-W008806
N-(3-Oxohexanoyl)-L-homoserine lactone -
HY-114816R
N-Butanoyl-L-homoserine lactone (Standard) -
HY-108700
N-3-Oxo-octanoyl-L-homoserine lactone -
HY-128200
Pseudomonas quinolone signal -
HY-W019543
Disuccinimidyl suberate Crosslinker -
HY-D0975
Sulfo-SMCC sodium -
HY-133685R
N-Hexanoyl-L-homoserine lactone (Standard)
Publications Citing Use of MedChemExpress (MCE) N-Butanoyl-L-homoserine lactone
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Biological Activity
Description
IC50 & Target
[1]|
Cleavable Linker |
In Vitro
The dissociation constant (Kd value) of aptamer binding to N-Butanoyl-L-homoserine lactone (C4-HSL) is 28.47 nM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 67605-85-0
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Appearance Solid
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Molecular Weight 171.20
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Formula C8H13NO3
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Color White to off-white
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SMILES
CCCC(N[C@H]1CCOC1=O)=O
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Synonyms
C4-HSL; N-Butyryl-L-homoserine lactone
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Microb Pathog
YXL-13 attenuates virulence in Pseudomonas aeruginosa mutants and clinical isolates by inhibiting quorum sensing regulators LasR and RhlR. [Abstract]2026 Mar:212:108298. PMID: 41520905 -
BMC Infect Dis
Harmine acts as a quorum sensing inhibitor decreasing the virulence and antibiotic resistance of Pseudomonas aeruginosa. [Abstract]2024 Jul 31;24(1):760. PMID: 39085766 -
J Antibiot (Tokyo)
Repurposing Tirazone as an effective quorum-sensing inhibitor against Pseudomonas aeruginosa virulence and biofilm formation. [Abstract]2026 Apr;79(4):248-263. PMID: 41708876
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (584.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 20 mg/mL (116.83 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.8413 mL | 29.2063 mL | 58.4126 mL | 146.0315 mL |
| 5 mM | 1.1683 mL | 5.8413 mL | 11.6825 mL | 29.2063 mL | |
| 10 mM | 0.5841 mL | 2.9206 mL | 5.8413 mL | 14.6031 mL | |
| 15 mM | 0.3894 mL | 1.9471 mL | 3.8942 mL | 9.7354 mL | |
| 20 mM | 0.2921 mL | 1.4603 mL | 2.9206 mL | 7.3016 mL | |
| 25 mM | 0.2337 mL | 1.1683 mL | 2.3365 mL | 5.8413 mL | |
| 30 mM | 0.1947 mL | 0.9735 mL | 1.9471 mL | 4.8677 mL | |
| 40 mM | 0.1460 mL | 0.7302 mL | 1.4603 mL | 3.6508 mL | |
| 50 mM | 0.1168 mL | 0.5841 mL | 1.1683 mL | 2.9206 mL | |
| 60 mM | 0.0974 mL | 0.4868 mL | 0.9735 mL | 2.4339 mL | |
| 80 mM | 0.0730 mL | 0.3651 mL | 0.7302 mL | 1.8254 mL | |
| 100 mM | 0.0584 mL | 0.2921 mL | 0.5841 mL | 1.4603 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Butanoyl-L-homoserine lactone Related Classifications
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