C4-HSL aptamers for blocking qurom sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysis

  • PLoS One. 2019 Feb 19;14(2):e0212041. doi: 10.1371/journal.pone.0212041.
Meng Zhao  1  2 ,  Weibin Li  2 ,  Kuancan Liu  2 ,  Huiling Li  2 ,  Xiaopeng Lan  1  2
Affiliations
  • 1. Second Military Medical University, Shanghai, China.
  • 2. Institute for Laboratory Medicine, The 900th Hospital of Joint Service Support Force, Fuzhou, Fujian, China.
PMID: 30779754 DOI: 10.1371/journal.pone.0212041
Abstract

This study aimed to screen DNA Aptamers against the signal molecule C4-HSL of the rhl system for the inhibition of biofilm formation of Pseudomonas aeruginosa using an improved systematic evolution of ligand by exponential enrichment (SELEX) method based on a structure-switching fluorescent activating bead. The Aptamers against the C4-HSL with a high affinity and specifity were successfully obtained and evaluated in real-time by this method. Results of biofilm inhibition experiments in vitro showed that the biofilm formation of P. aeruginosa was efficiently reduced to about 1/3 by the Aptamers compared with that of the groups without the Aptamers. Independent secondary structure simulation and computer-aided tertiary structure prediction (3dRNA) showed that the Aptamers contained a highly conserved Y-shaped structural unit. Therefore, this study benefits the search for new methods for the detection and treatment of P. aeruginosa biofilm formation.

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