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Cat. No.: HY-108541

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N2-Methyl-L-arginine

N2-Methyl-L-arginine is a selective L-arginine uptake inhibitor, with a K_i value of 500 μM.

For research use only. We do not sell to patients.

CAS No.: 2480-28-6
Formula: C₇H₁₆N₄O₂
Molecular Weight:188.23

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (263 KB)

N2-Methyl-L-arginine Chemical Structure

CAS No.: 2480-28-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

N2-Methyl-L-arginine is a selective L-arginine uptake inhibitor, with a K_i value of 500 μM^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Macrophage	IC₅₀	28 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in ddY mouse peritoneal macrophages by Griess reagent method Inhibition of LPS-induced NO production in ddY mouse peritoneal macrophages by Griess reagent method	[PMID: 12542351]
Macrophage	IC₅₀	64.24 μM Compound: L-NMMA	Inhibition of LPS-stimulated nitric oxide production in BALB/c mouse macrophages after 20 hrs by Griess assay relative to control Inhibition of LPS-stimulated nitric oxide production in BALB/c mouse macrophages after 20 hrs by Griess assay relative to control	[PMID: 18723353]
Peritoneal macrophage cell	IC₅₀	36 μM Compound: L-NMMA	Hepatoprotective activity in ddY mouse peritoneal macrophages assessed as inhibition of LPS-induced nitric oxide production after 20 hrs by Griess method relative to untreated control Hepatoprotective activity in ddY mouse peritoneal macrophages assessed as inhibition of LPS-induced nitric oxide production after 20 hrs by Griess method relative to untreated control	[PMID: 19775895]
RAW264.7	IC₅₀	39.2 μM Compound: N-monomethyl-L-arginine	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production after 24 hrs by Griess assay	[PMID: 24547740]
RAW264.7	IC₅₀	44 μM Compound: L-NMMA (N-monomethyl arginine)	Inhibitory activity against nitric oxide production in lipopolysaccharide-activated mouse macrophage RAW264.7 cells Inhibitory activity against nitric oxide production in lipopolysaccharide-activated mouse macrophage RAW264.7 cells	[PMID: 14552759]
Sf9	IC₅₀	0.65 μM Compound: L-NMMA	Inhibition of human eNOS expressed in baculovirus-infected insect sf9 cells assessed as conversion of [3H]-L-arginine to [3H]-L-citrulline preincubated for 15 mins with compound measured after 45 mins by scintillation counting Inhibition of human eNOS expressed in baculovirus-infected insect sf9 cells assessed as conversion of [3H]-L-arginine to [3H]-L-citrulline preincubated for 15 mins with compound measured after 45 mins by scintillation counting	[PMID: 22420844]
Sf9	IC₅₀	0.95 μM Compound: L-NMMA	Inhibition of human nNOS expressed in baculovirus-infected insect sf9 cells assessed as conversion of [3H]-L-arginine to [3H]-L-citrulline preincubated for 15 mins with compound measured after 45 mins by scintillation counting Inhibition of human nNOS expressed in baculovirus-infected insect sf9 cells assessed as conversion of [3H]-L-arginine to [3H]-L-citrulline preincubated for 15 mins with compound measured after 45 mins by scintillation counting	[PMID: 22420844]
Sf9	IC₅₀	1.8 μM Compound: L-NMMA	Inhibition of human iNOS expressed in baculovirus-infected insect sf9 cells assessed as conversion of [3H]-L-arginine to [3H]-L-citrulline preincubated for 15 mins with compound measured after 45 mins by scintillation counting Inhibition of human iNOS expressed in baculovirus-infected insect sf9 cells assessed as conversion of [3H]-L-arginine to [3H]-L-citrulline preincubated for 15 mins with compound measured after 45 mins by scintillation counting	[PMID: 22420844]

Chemical Information

CAS No. 2480-28-6
Molecular Weight 188.23
Formula C₇H₁₆N₄O₂
SMILES

N=C(N)NCCC[C@@H](C(O)=O)NC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (263 KB)
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Pisoni RL, et al. Important differences in cationic amino acid transport by lysosomal system c and system y+ of the human fibroblast. J Biol Chem. 1987 Nov 5;262(31):15011-8. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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