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Cat. No.: HY-175424

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N3-CMC

N3-CMC is a click chemistry reagent. N3-CMC containing an azide group can undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups. N3-CMC can also undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. N3-CMC reacts specifically with the pseudouridine (Ψ)-containing RNA, and biotin is subsequently conjugated to the N3-CMC-Ψ RNA via click chemistry, quantitatively analyzing the dynamic changes of Ψ.

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CAS No.: 1801187-61-0
Formule: C₂₁H₃₂N₆O₄S
Masse moléculaire:464.58

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (267 KB)

N3-CMC Chemical Structure

CAS No.: 1801187-61-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

Click chemistry is used to describe the selective, modular, wide range and high-yield chemical reactions that allow rapid synthesis of new compounds through heteroatom linking (C-X-C). Bertozzi developed click chemistry in a new dimension—Bioorthogonal chemistry. It is defined as a rapid and selective reaction that does not interfere with biological processes under physiological conditions. Common reaction structures of click chemistry, such as Azide, Alkyne, DBCO, BCN, TCO and Tetrazine.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1801187-61-0
Masse moléculaire 464.58
Formule C₂₁H₃₂N₆O₄S
SMILES

C[N+]1(CCN=[C@@]=N[C@H]2CC[C@@H](N=[N+]=[N-])CC2)CCOCC1.O=S(C3=CC=C(C)C=C3)([O-])=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (267 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Li X, et al. Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome. Nat Chem Biol. 2015 Aug;11(8):592-7. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

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100 mg

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