Pentafluorobenzenesulfonyl fluorescein

Name: Pentafluorobenzenesulfonyl fluorescein
Brand: MedChemExpress
SKU: HY-120972
Rating: 4.5 (1 reviews)

Cat. No.: HY-120972 Purity: 98.30%: Data Sheet
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Pentafluorobenzenesulfonyl fluorescein is a H₂O₂-selective sensor that can be used to detect H₂O₂ levels in cells. Pentafluorobenzenesulfonyl fluorescein is generally non-fluorescent, but emits fluorescence when its sulfonyl bond undergoes perhydrolysis by H₂O₂. Pentafluorobenzenesulfonyl fluorescein undergoes slight cleavage of its sulfonate ester bond by [Cu (phen)₂]²⁺, and can detect hydrogen peroxide around the ablation sites of fin tissues and keratinocytes in zebrafish larvae.

For research use only. We do not sell to patients.

Pentafluorobenzenesulfonyl fluorescein Chemical Structure

CAS No. : 728912-45-6

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1 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

In Vivo

Pentafluorobenzenesulfonyl fluorescein (4µM; incubated in media; single 1-2 hour exposure prior to imaging) detects significantly increased hydrogen peroxide levels in the caudal fin of glucose-treated larval Danio rerio, with elevated regional fluorescence at 5dpf and widespread, intensified fluorescence at 8dpf^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nacre, Tuebingen, Tg(isl2b:GFP), Tg(NF-κB:GFP) (larval stage, 2-8 days post fertilization)^[1]
Dosage:	4 µM
Administration:	incubated in media; single 1-2 hour exposure prior to imaging
Result:	Detected fluorescence (indicating hydrogen peroxide) in 40-60% of glucose-treated larvae's distal caudal fin at 5dpf, a rate higher than in untreated larvae. Showed widespread fluorescence in glucose-treated larvae's caudal fin at 8dpf, with significantly higher fluorescence intensity compared to untreated larvae.

Molecular Weight

562.42

Formula

C₂₆H₁₁F₅O₇S

CAS No.

728912-45-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

FC1=C(C(F)=C(C(F)=C1S(OC2=CC=C3C4(C5=C(OC3=C2)C=C(O)C=C5)OC(C6=CC=CC=C64)=O)(=O)=O)F)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Purity & Documentation

Purity: 98.3%

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Data Sheet (274 KB) SDS (252 KB)

COA (253 KB)

Handling Instructions (2659 KB)

References

[1]. Waldron AL, et al. Oxidative stress-dependent MMP-13 activity underlies glucose neurotoxicity. J Diabetes Complications. 2018 Mar;32(3):249-257.

[2]. Leichnitz S, et al. A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases. Chem Commun (Camb). 2018;54(95):13411-13414.

[3]. Rieger S, et al. Hydrogen peroxide promotes injury-induced peripheral sensory axon regeneration in the zebrafish skin. PLoS Biol. 2011;9(5):e1000621.