Pentafluorobenzenesulfonyl fluorescein
Based on 1 Customer Validation
Pentafluorobenzenesulfonyl fluorescein is a H2O2-selective sensor that can be used to detect H2O2 levels in cells. Pentafluorobenzenesulfonyl fluorescein is generally non-fluorescent, but emits fluorescence when its sulfonyl bond undergoes perhydrolysis by H2O2. Pentafluorobenzenesulfonyl fluorescein undergoes slight cleavage of its sulfonate ester bond by [Cu (phen)2]2+, and can detect hydrogen peroxide around the ablation sites of fin tissues and keratinocytes in zebrafish larvae.
For research use only. We do not sell to patients.
- Purity: 98.30%
- CAS No.: 728912-45-6
- Formula: C26H11F5O7S
- Molecular Weight:562.42
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nacre, Tuebingen, Tg(isl2b:GFP), Tg(NF-κB:GFP) (larval stage, 2-8 days post fertilization)[1]
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Dosage:4 µM
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Administration:incubated in media; single 1-2 hour exposure prior to imaging
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Result:Detected fluorescence (indicating hydrogen peroxide) in 40-60% of glucose-treated larvae's distal caudal fin at 5dpf, a rate higher than in untreated larvae.
Showed widespread fluorescence in glucose-treated larvae's caudal fin at 8dpf, with significantly higher fluorescence intensity compared to untreated larvae.
Chemical Information
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CAS No. 728912-45-6
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Appearance Solid
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Molecular Weight 562.42
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Formula C26H11F5O7S
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Color Light yellow to yellow
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SMILES
FC1=C(C(F)=C(C(F)=C1S(OC2=CC=C3C4(C5=C(OC3=C2)C=C(O)C=C5)OC(C6=CC=CC=C64)=O)(=O)=O)F)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Leichnitz S, et al. A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases. Chem Commun (Camb). 2018;54(95):13411-13414. [Content Brief]
[3]. Rieger S, et al. Hydrogen peroxide promotes injury-induced peripheral sensory axon regeneration in the zebrafish skin. PLoS Biol. 2011;9(5):e1000621. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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