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Cat. No.: HY-E70528

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HRV-3C protease fuses with GST

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HRV-3C protease fuses with GST is a recombinant protease fused with HRV-3C protease and GST, which recognizes the LEVLFQGP polypeptide sequence. HRV-3C protease fuses with GST precisely cleaves between glutamine and glycine-proline residues to remove fusion tags from target proteins. HRV-3C protease fuses with GST exhibits cleavage activity both in vitro in silkworm fat body lysates and in vivo in silkworm larval fat bodies, and achieves functional expression in E. coli and silkworm-baculovirus expression systems. HRV-3C protease fuses with GST can be applied to recombinant protein-related research.

For research use only. We do not sell to patients.

CAS No.: 2178107-79-2

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

HRV-3C protease fuses with GST Chemical Structure

CAS No.: 2178107-79-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

GST-HRV-3C protease fuses with GST (1:1-1:2 (v/v) ratio; 4 h) expressed in Escherichia coli Rosetta-gami B cells potently cleaves its target substrate GST-3Csite-VP15^[1].
GST-HRV-3C protease fuses with GST (1:1-1:3 (v/v) ratio; 4 h) expressed in silkworm (Bombyx mori) larvae fat bodies cleaves its target substrate GST-3Csite-VP15-FLAG, with more complete cleavage observed at higher protease lysate ratios^[1].
GST-HRV 3C protease (1:50 (w/w) ratio; 6 mM DTT; 0.5 mM EDTA; 1-16 h; 4 °C, 25 °C) actively cleaves the GST-Cyclin A fusion protein, and maintains efficient proteolytic activity across PBS, Tris-HCl (pH 7.5, 8), and HEPES (pH 7.5, 8) buffer systems^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2178107-79-2
Appearance Liquid
Color Colorless to light yellow
SMILES

[HRV-3C protease fuses with GST]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (265 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xu J, et al. In vivo enzymatic digestion of HRV 3C protease cleavage sites-containing proteins produced in a silkworm-baculovirus expression system. Biosci Rep. 2022 Jun 30;42(6):BSR20220739.

[2]. Antoniou G, et al. Optimization of Soluble Expression and Purification of Recombinant Human Rhinovirus Type-14 3C Protease Using Statistically Designed Experiments: Isolation and Characterization of the Enzyme. Mol Biotechnol. 2017;59(9-10):407-424. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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