HRV-3C protease fuses with GST
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HRV-3C protease fuses with GST is a recombinant protease fused with HRV-3C protease and GST, which recognizes the LEVLFQGP polypeptide sequence. HRV-3C protease fuses with GST precisely cleaves between glutamine and glycine-proline residues to remove fusion tags from target proteins. HRV-3C protease fuses with GST exhibits cleavage activity both in vitro in silkworm fat body lysates and in vivo in silkworm larval fat bodies, and achieves functional expression in E. coli and silkworm-baculovirus expression systems. HRV-3C protease fuses with GST can be applied to recombinant protein-related research.
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- No. CAS: 2178107-79-2
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Actividad biológica
Descripciòn
In Vitro
GST-HRV-3C protease fuses with GST (1:1-1:2 (v/v) ratio; 4 h) expressed in Escherichia coli Rosetta-gami B cells potently cleaves its target substrate GST-3Csite-VP15[1].
GST-HRV-3C protease fuses with GST (1:1-1:3 (v/v) ratio; 4 h) expressed in silkworm (Bombyx mori) larvae fat bodies cleaves its target substrate GST-3Csite-VP15-FLAG, with more complete cleavage observed at higher protease lysate ratios[1].
GST-HRV 3C protease (1:50 (w/w) ratio; 6 mM DTT; 0.5 mM EDTA; 1-16 h; 4 °C, 25 °C) actively cleaves the GST-Cyclin A fusion protein, and maintains efficient proteolytic activity across PBS, Tris-HCl (pH 7.5, 8), and HEPES (pH 7.5, 8) buffer systems[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2178107-79-2
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[HRV-3C protease fuses with GST]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
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Ficha de datos (265 KB)
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SDS (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HRV-3C protease fuses with GST Related Classifications
HY-E70528 Related Classifications
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