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Cat. No.: HY-174233

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PROTAC SARS-CoV-2 Mpro degrader-4

PROTAC SARS-CoV-2 Mpro degrader-4 is a SARS-CoV-2 M^Pro PROTAC degrader with antiviral activity, with a DC₅₀ value of 4.7 μM. PROTAC SARS-CoV-2 Mpro degrader-4 induces M^Pro ubiquitination and proteasomal degradation, and inhibits SARS-CoV-2 replication. PROTAC SARS-CoV-2 Mpro degrader-4 can be used in the research of coronavirus infections.
(Pink: SARS-CoV ligand (HY-32717); Blue: Cereblon ligand (HY-42771); Black: linker (HY-42149)).

For research use only. We do not sell to patients.

Formula: C₄₀H₃₇ClFN₇O₁₀
Molecular Weight:830.21

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

PROTAC SARS-CoV-2 Mpro degrader-4 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

PROTAC SARS-CoV-2 Mpro degrader-4 is a SARS-CoV-2 M^Pro PROTAC degrader with antiviral activity, with a DC₅₀ value of 4.7 μM. PROTAC SARS-CoV-2 Mpro degrader-4 induces M^Pro ubiquitination and proteasomal degradation, and inhibits SARS-CoV-2 replication. PROTAC SARS-CoV-2 Mpro degrader-4 can be used in the research of coronavirus infections^[1]. (Pink: SARS-CoV ligand (HY-32717); Blue: Cereblon ligand (HY-42771); Black: linker (HY-42149)).

All PROTACs Isoforms :

In Vitro

PROTAC SARS-CoV-2 Mpro degrader-4 (LLP019) (0.1-100 μM; 24 h) dose-dependently degrades M^Pro protein in HEK293F cells without obvious cytotoxicity^[1].
PROTAC SARS-CoV-2 Mpro degrader-4 (25 μM; 5 h) degrades approximately 40% of M^Pro protein in HEK293F cells co-treated with Cycloheximide (HY-12320)^[1].
PROTAC SARS-CoV-2 Mpro degrader-4 (12.5-50 μM; 48 h) potently inhibits the replication of SARS-CoV-2 (BavPat1/2020) and SARS-CoV-2 Delta variant (FFM-IND8424/2021) in Calu3 cells, but only partially inhibits SARS-CoV replication and shows no inhibitory effect on MERS-CoV replication^[1].
PROTAC SARS-CoV-2 Mpro degrader-4 (25 μM; 5 h) exerts a ubiquitin-proteasome system (UPS)-dependent M^Pro protein degradation effect in HEK293F cells co-treated with Pevonedistat (HY-70062) or TAK243 (TAK-243), with degradation being inhibited^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK293F
Concentration:

195 nM, 390 nM, 780 nM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM, 50 μM, 100 μM
Incubation Time:

24 h
Result:

Degraded ectopically expressed MPro in a dose-dependent manner, exhibiting maximal degradation up to 50 μM, and showing a hook effect with reversed degradation activity at 100 μM.

Western Blot Analysis^[1]

Cell Line:

HEK293F
Concentration:

25 μM
Incubation Time:

5 h (in the presence of cycloheximide and pevonedistat or TAK243)
Result:

The MPro degradation induced by PROTAC SARS-CoV-2 Mpro degrader-4 was blocked by the NEDD8-activating enzyme inhibitor pevonedistat and the ubiquitin-activating enzyme inhibitor TAK243, indicating that the degradation is dependent on the host's ubiquitin-proteasome system (UPS).

Chemical Information

Molecular Weight 830.21
Formula C₄₀H₃₇ClFN₇O₁₀
SMILES

CCOC1=C(NC(CCOCCOCCNC(COC2=C(C(N(C3CCC(NC3=O)=O)C4=O)=O)C4=CC=C2)=O)=O)C=C(C(NC5=CC(Cl)=C(F)C=C5)=C(C#N)C=N6)C6=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Veeck, et al. "Discovery of an antiviral PROTAC targeting the SARS-CoV-2 main protease using an allosteric warhead." bioRxiv (2025): 2025-05.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References

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PROTAC SARS-CoV-2 Mpro degrader-4

All PROTACs Isoforms