Pluripotent stem cell culture is a technology that cultivates and maintains multi-lineage differentiation potential of pluripotent stem cells (such as embryonic stem cells or induced pluripotent stem cells) in vitro. This culture system allows stem cells to maintain their self-renewal and pluripotent differentiation properties and can differentiate into various cell types, such as nerve cells, heart cells, liver cells, etc. Multifunctional stem cell culture has potential clinical applications in areas such as biomedical research, tissue engineering, and drug testing.