PTK7/β-catenin-IN-6
Based on 1 Customer Validation
PTK7/β-catenin-IN-6 (Compound 03653) is an inhibitor for PTK7/β-catenin interaction, which inhibits the Wnt signaling pathway, and exhibits an anticancer activity against colorectal cancer (CRC).
For research use only. We do not sell to patients.
- CAS No.: 1219439-82-3
- Formula: C25H19Cl2N3O3
- Molecular Weight:480.34
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PTK7/β-catenin-IN-6 (25 μM) misregulates the β-catenin responsive transcription and inihibits the Wnt signaling pathway through regulating the mRNA expressions of Wnt signaling related genes, which are associated with cell proliferation and migration[1].
PTK7/β-catenin-IN-6 (0-100 μM) arrests the cell cycle in G0/G1 phase through block of CyclinA2, CyclinE2 and CDK production, inhibits proliferations of HCT116, SW480 and MEF with IC50s of 20.1, 19.6 and 46.3 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116, SW480 and MEF
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Concentration:0-100 μM
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Incubation Time:24 h
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Result:Inhibited proliferations of cells HCT116, SW480 and MEF.
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Cell Line:HCT116, SW480
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Concentration:0-100 μM
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Incubation Time:24 h
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Result:Decreased levels of CyclinA2, CyclinE2 and CDK.
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Cell Line:HCT116, SW480
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Concentration:25 μM
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Incubation Time:24 h
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Result:Increased mRNA levels of PLAUR, FOSL1 and NRP1.
Decreased mRNA levels of AXIN2.
Chemical Information
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CAS No. 1219439-82-3
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Appearance Solid
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Molecular Weight 480.34
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Formula C25H19Cl2N3O3
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SMILES
O=C(N1C2=C(NC(C1CC(NC3=CC=C(C(Cl)=C3)Cl)=O)=O)C=CC=C2)/C=C/C4=CC=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 73.33 mg/mL (152.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (5.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0819 mL | 10.4093 mL | 20.8186 mL | 52.0465 mL |
| 5 mM | 0.4164 mL | 2.0819 mL | 4.1637 mL | 10.4093 mL | |
| 10 mM | 0.2082 mL | 1.0409 mL | 2.0819 mL | 5.2046 mL | |
| 15 mM | 0.1388 mL | 0.6940 mL | 1.3879 mL | 3.4698 mL | |
| 20 mM | 0.1041 mL | 0.5205 mL | 1.0409 mL | 2.6023 mL | |
| 25 mM | 0.0833 mL | 0.4164 mL | 0.8327 mL | 2.0819 mL | |
| 30 mM | 0.0694 mL | 0.3470 mL | 0.6940 mL | 1.7349 mL | |
| 40 mM | 0.0520 mL | 0.2602 mL | 0.5205 mL | 1.3012 mL | |
| 50 mM | 0.0416 mL | 0.2082 mL | 0.4164 mL | 1.0409 mL | |
| 60 mM | 0.0347 mL | 0.1735 mL | 0.3470 mL | 0.8674 mL | |
| 80 mM | 0.0260 mL | 0.1301 mL | 0.2602 mL | 0.6506 mL | |
| 100 mM | 0.0208 mL | 0.1041 mL | 0.2082 mL | 0.5205 mL |
PTK7/β-catenin-IN-6 Related Classifications
HY-160493 Related Classifications
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