LNA for optimization of fluorescent oligonucleotide probes: improved spectral properties and target binding

  • Bioconjug Chem. 2011 Apr 20;22(4):533-9. doi: 10.1021/bc1005027.
Irina V Astakhova  1 ,  Alexey V Ustinov ,  Vladimir A Korshun ,  Jesper Wengel
Affiliations
  • 1. Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark , DK-5230 Odense M, Denmark.
PMID: 21401111 DOI: 10.1021/bc1005027
Abstract

Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.

Products