In vitro cytotoxicity and genotoxicity of Furia®180 SC (zeta-cypermethrin) and Bulldock 125®SC (β-cyfluthrin) pyrethroid insecticides in human peripheral blood lymphocytes

  • Toxicol Mech Methods. 2018 May;28(4):268-278. doi: 10.1080/15376516.2017.1402977.
María Elena Calderón-Segura  1 ,  Sandra Gómez-Arroyo  1 ,  Josefina Cortés-Eslava  1 ,  Carmen Martínez-Valenzuela  2 ,  Luis Humberto Mojica-Vázquez  1 ,  Magdiel Sosa-López  1 ,  Diana Flores-Ramírez  1 ,  Zaira Edtih Romero-Velázquez  1
Affiliations
  • 1. a Genotoxicología Ambiental, Departamento de Ciencias Ambientales , Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México , Mexico.
  • 2. b Instituto de Investigación en Ambiente y Salud , Universidad de Occidente , Los Mochis , Sinaloa , Mexico.
PMID: 29129129 DOI: 10.1080/15376516.2017.1402977
Abstract

In the present study, human peripheral blood lymphocytes were exposed in vitro to 0, 6, 12, 18, 24, and 30 μg/mL Furia®180 SC (zeta-cypermethrin) and 0, 6.3, 12.5, 18.8, 25, and 31.3 μg/mL Bulldock®125 SC (β-cyfluthrin). Exposure to 32 µg/mL bleomycin for 24 h served as a positive control. The cytotoxic and genotoxic effects of each Insecticide were analyzed using alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated through three genotoxicity parameters: tail length (TL), tail moment (TM) and tail intensity (TI). Furia®180 SC and Bulldock®125 SC pyrethroid insecticides and bleomycin significantly increased DNA damage in a concentration-dependent manner. Bulldock®125 SC induced more DNA damage than Furia. Lymphocyte viability did not change after exposure to different concentrations of the two pyrethroid insecticides and bleomycin. Moreover, genotoxic results demonstrated that Furia®180 SC and Bulldock®125 SC insecticides caused in vitro DNA damage in human peripheral lymphocytes.

Keywords
Genotoxicity; alkaline comet assay; cytotoxicity; human peripheral blood lymphocytes; zeta-cypermethrin and β-cyfluthrin pyrethroid insecticides.
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