Kaempferol-3-o-β-d-glucuronate exhibit potential anti-inflammatory effect in LPS stimulated RAW 264.7 cells and mice model

  • Int Immunopharmacol. 2018 Apr;57:62-71. doi: 10.1016/j.intimp.2018.01.041.
Vidushi Khajuria  1 ,  Shilpa Gupta  1 ,  Neha Sharma  2 ,  Harshita Tiwari  3 ,  Subhash Bhardwaj  4 ,  Prabhu Dutt  2 ,  Naresh Satti  2 ,  Amit Nargotra  5 ,  Asha Bhagat  6 ,  Zabeer Ahmed  7
Affiliations
  • 1. Academy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India; Inflammation Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu Tawi, Jammu and Kashmir, India.
  • 2. Natural Product Chemistry, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India.
  • 3. Academy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India; Discovery Informatics Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India.
  • 4. Department of Pathology, Government Medical College, Jammu Tawi, Jammu and Kashmir, India.
  • 5. Discovery Informatics Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India.
  • 6. Inflammation Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu Tawi, Jammu and Kashmir, India.
  • 7. Academy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India. Electronic address: [email protected].
PMID: 29475097 DOI: 10.1016/j.intimp.2018.01.041
Abstract

Kaempferol-3-O-β-d-glucuronide (K3G) having various pharmacological effects was explored for its anti-inflammatory effect in LPS induced RAW 264.7 cells and mice model. K3G significantly inhibited various pro-inflammatory mediators like IL-1β, NO, PGE2, and LTB4. It upregulated the secretion of anti-inflammatory cytokine IL-10. K3G is found to reduce inflammation when studied for parameters like phagocytic index, carrageenan induced paw edema in mice and organ weight. It reduced inflammation in a dose dependent manner both in-vitro and in-vivo. Further molecular insights into the study reveal that K3G blocks the phosphorylation of NF-kB which is key regulator of inflammation, thereby exhibiting anti-inflammatory potential. Hence, this study permits further investigation to develop K3G as anti-inflammatory drug.

Keywords
Anti-inflammatory; Histopathology; Pathway elucidation; Proinflammatory.
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