Corynoline Exhibits Anti-inflammatory Effects in Lipopolysaccharide (LPS)-Stimulated Human Umbilical Vein Endothelial Cells through Activating Nrf2

  • Inflammation. 2018 Oct;41(5):1640-1647. doi: 10.1007/s10753-018-0807-6.
Bin Liu  1 ,  Kai Su  2 ,  Jiaxin Wang  3 ,  Jingjing Wang  3 ,  Zhuoyuan Xin  4 ,  Fan Li  4 ,  Yunhe Fu  5  6
Affiliations
  • 1. Cardiovascular Disease Center, First Hospital of Jilin University, Changchun, Jilin Province, 130021, People's Republic of China.
  • 2. Department of Otolaryngology Head and Neck Surgery, Second Hospital of Jilin University, Changchun, Jilin Province, 130041, People's Republic of China.
  • 3. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
  • 4. Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
  • 5. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China. [email protected].
  • 6. Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China. [email protected].
PMID: 29748730 DOI: 10.1007/s10753-018-0807-6
Abstract

Corynoline, a bioactive compound isolated from Corydalis bungeana Turcz., has been known to have anti-inflammatory activity. However, its effects on the inflammation of the cardiovascular system have not been reported yet. The aim of this study was to investigate the anti-inflammatory effects of corynoline on lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs). The results showed that LPS significantly increased the expression of VCAM-1 and ICAM-1. The production of cytokines TNF-α and IL-8 was also up-regulated by LPS. However, these increases were concentration-dependently suppressed by the treatment of corynoline. To investigate the anti-inflammatory mechanism of corynoline, we checked the activation of NF-κB and the expression of Nrf2. The results showed that LPS-induced NF-κB activation was suppressed by corynoline. The expression of Nrf2 and HO-1 was up-regulated by the treatment of corynoline. Knockdown of Nrf2 could reverse the anti-inflammatory effects of corynoline. In conclusion, the results indicated that corynoline exhibited anti-inflammatory activity by activating Nrf2.

Keywords
NF-κB; Nrf2; TNF-α; corynoline; human umbilical vein endothelial cells.
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