Stability and properties of Z-DNA containing artificial nucleobase 2'-O-methyl-8-methyl guanosine

  • Bioorg Med Chem. 2019 Jan 15;27(2):364-369. doi: 10.1016/j.bmc.2018.12.005.
Thananjeyan Balasubramaniyam  1 ,  Takumi Ishizuka  1 ,  Yan Xu  2
Affiliations
  • 1. Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyo-take, Miyazaki 889-1692, Japan.
  • 2. Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyo-take, Miyazaki 889-1692, Japan. Electronic address: [email protected].
PMID: 30545733 DOI: 10.1016/j.bmc.2018.12.005
Abstract

We synthesized several DNA oligonucleotides containing one or several 2'-O-methyl-8-methyl guanosine (m8Gm) and demonstrated that these oligonucleotides not only stabilize the Z-DNA with a wide range of sequences under low salt conditions but also possess high thermal stability. Using artificial nucleobase-containing oligonucleotides, we studied the interaction of the Zα domain with Z-DNA. Furthermore, we showed that the m8Gm-contained oligonucleotides allow to study the photochemical reaction of Z-DNA.

Keywords
Artificial nucleobase; Circular dichroism; Interaction of protein and Z-DNA; Photochemical reaction; Z-DNA oligonucleotide.
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