Diterpenoids from the seeds of Euphorbia lathyris and their effects on microglial nitric oxide production

  • Fitoterapia. 2021 Apr:150:104834. doi: 10.1016/j.fitote.2021.104834.
Quan Zuo  1 ,  Hong-Yan Mu  1 ,  Qi Gong  2 ,  Xun Ding  3 ,  Wei Wang  2 ,  Hai-Yan Zhang  4 ,  Wei-Min Zhao  5
Affiliations
  • 1. Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, People's Republic of China.
  • 2. CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
  • 3. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, People's Republic of China; CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
  • 4. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, People's Republic of China; CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China. Electronic address: [email protected].
  • 5. Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, People's Republic of China. Electronic address: [email protected].
PMID: 33513430 DOI: 10.1016/j.fitote.2021.104834
Abstract

Four new lathyrane-type Diterpenoids (1-4) and a novel macrocyclic diterpenoid (5) featuring a 5/7/7/4-fused ring system, together with seventeen known ones (6-22), were isolated from the seeds of Euphorbia lathyris. Their structures were elucidated by extensive spectroscopic analyses and single crystal X-ray crystallography. These isolates were evaluated for their inhibition against nitric oxide (NO) production induced by lipopolysaccharide (LPS) in BV-2 microglial cells. As a result, the inhibitory rates of compounds 1, 3, 4, 6, 7, 9-11, 13-15, 20, and 21 on NO production were more than 40% with the cell viability more than 80% at their effective concentrations. In addition, compounds 6 and 11 markedly reduced the mRNA levels of pro-inflammatory cytokines IL-6 and IL-1β in LPS-stimulated BV-2 cells.

Keywords
Anti-inflammatory; Configuration; Diterpenoids; Euphorbia lathyris; NO production.
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