Triterpene saponins from the roots of Acacia senegal (L.) Willd

  • Fitoterapia. 2021 Jun:151:104859. doi: 10.1016/j.fitote.2021.104859.
Abdou Tchoukoua  1 ,  Mireille Heti Douanla  2 ,  Nanang Rudianto Ariefta  3 ,  Jun Yoshida  4 ,  Yoshiaki Ito  5 ,  Bonaventure Tchaleu Ngadjui  6 ,  Yoshihito Shiono  7
Affiliations
  • 1. Department of Chemistry, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon. Electronic address: [email protected].
  • 2. Department of Organic Chemistry, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon.
  • 3. Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan.
  • 4. Center for Liberal Arts and Sciences, Iwate Medical University, Yahaba, Iwate 028-3694, Japan.
  • 5. Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan.
  • 6. Department of Organic Chemistry, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon. Electronic address: [email protected].
  • 7. Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan. Electronic address: [email protected].
PMID: 33610746 DOI: 10.1016/j.fitote.2021.104859
Abstract

Six new triterpenoid saponins, named senegalosides A-F (1-6) were isolated from the seedpods and roots of Acacia senegal (Mimosaceae). Their structures were elucidated using 1D and 2D-NMR spectroscopic analysis and mass spectrometry. Compound 1 possesses an unusual sapogenin, 3β-hydroxy-21-oxo-olean-12-en-28-oic acid (machaeric acid), and was reported here in its natural form for the first time within the genus Acacia. Senegaloside A is the first example of a machaeric-type triterpenoid glycoside in the plant kingdom. The cytotoxic effect of isolated saponins was evaluated on the H4IIE rat hepatoma cell line. As a result, compounds 1, 3-6 were not significantly cytotoxic to H4IIE cells even at 200 μM. Compound 2 was suppressed cell viability at 50-200 μM.

Keywords
Acacia senegal; Cytotoxicity; H4IIE hepatoma cell line; Mimosaceae; Triterpenoid saponins.
Products