Design of "Off-On-Off" fluorescence sensors for Heparin detection by precise modulation of molecular structure

  • Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jun 8:320:124630. doi: 10.1016/j.saa.2024.124630.
Yanan Li  1 ,  Jian Cao  2 ,  Chuanxiao Wang  1 ,  Yiyi Qiao  1 ,  Jiayu Gao  1 ,  Xiao Zhang  1 ,  Le Wang  1
Affiliations
  • 1. School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, 333 Longteng Road, Shanghai 201620, PR China.
  • 2. School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, 333 Longteng Road, Shanghai 201620, PR China. Electronic address: [email protected].
PMID: 38865888 DOI: 10.1016/j.saa.2024.124630
Abstract

In this strategy, the fluorescence sensor Nap-Co-T1 employing the fluorescence resonance energy transfer (FRET) mechanism was designed and synthesized to have an efficient response to Heparin, and the FRET mechanism was explored for different excitation-emission wavelengths with different distances between the energy acceptor and the energy donor (comparing with fluorescence sensor Nap-TPA-T2). Upon the addition of Heparin, the fluorescence emission of Nap-Co-T1 was turned on at 565 nm, and the fluorescence color changed of the solution from colorless to bright yellow. The limit of detection (LOD) was as low as 0.04 μg/mL. With the addition of antagonistic protamine (PRTM) to the sensor complex with Heparin, the fluorescence emission was turned off to a certain extent, and the reversibility of the "off-on-off" system was maintained for five cycles or more. In addition, Nap-Co-T1 provides rapid and sensitive detection of Heparin in human serum albumin solution and artificial urine and is highly sensitive to environmental viscosity.

Keywords
Artificial urine; FRET mechanism; Fluorescence; Heparin; Sensor; Viscosity.
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