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  3. ASGR1/ASGPR1 Protein, Human (HEK293, His)

ASGR1/ASGPR1 Protein, Human (HEK293, His)

Cat. No.: HY-P7610
COA Handling Instructions

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells.

For research use only. We do not sell to patients.

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50 μg $285 In-stock
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  • Biological Activity

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Description

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells[1].

Background

Asialoglycoprotein receptor 1 (ASGR1) has long been recognized as a hepatic surface marker and has been used to identify circulating hepatocellular carcinoma cells, purify hPSC-derived hepatocyte-like cells (HLCs) and to demonstrate the efficiency of HLC differentiation from hPSCs. ASGR1 marks a subset of albumin-positive HLCs, which are more similar than unpurified cells to mature hepatocytes[1].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P07306 (Q62-L291)

Gene ID

432  [NCBI]

Molecular Construction
N-term
ASGR1 (Q62-L291)
Accession # P07306
6*His
C-term
Synonyms
rHuASGR1, His; Asialoglycoprotein Receptor 1, ASGR1,
AA Sequence

QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLLHHHHHH

Molecular Weight

Approximately 38.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

ASGR1/ASGPR1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ASGR1/ASGPR1 Protein, Human (HEK293, His)
Cat. No.:
HY-P7610
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