1. Recombinant Proteins
  2. Receptor Proteins
  3. ASGR1/ASGPR1 Protein, Human (HEK293, His)

ASGR1/ASGPR1 Protein, Human (HEK293, His)

Cat. No.: HY-P7610
Handling Instructions

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells[1].

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $95 In-stock
Estimated Time of Arrival: December 31
50 μg $285 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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ASGR1/ASGPR1 Protein, Human (HEK293, His) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

HEK 293

Accession

P07306 (Q62-L291)

Gene ID

432

Synonyms
rHuASGR1, His; Asialoglycoprotein Receptor 1, ASGR1,
AA Sequence

QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLLHHHHHH

Molecular Weight

Approximately 38.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity
Data is not available.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Asialoglycoprotein receptor 1 (ASGR1) has long been recognized as a hepatic surface marker and has been used to identify circulating hepatocellular carcinoma cells, purify hPSC-derived hepatocyte-like cells (HLCs) and to demonstrate the efficiency of HLC differentiation from hPSCs. ASGR1 marks a subset of albumin-positive HLCs, which are more similar than unpurified cells to mature hepatocytes[1].

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
ASGR1/ASGPR1 Protein, Human (HEK293, His)
Cat. No.:
HY-P7610
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