ASGR1/ASGPR1 Protein, Human (HEK293, His)

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells^[1].

Asialoglycoprotein receptor 1 (ASGR1) has long been recognized as a hepatic surface marker and has been used to identify circulating hepatocellular carcinoma cells, purify hPSC-derived hepatocyte-like cells (HLCs) and to demonstrate the efficiency of HLC differentiation from hPSCs. ASGR1 marks a subset of albumin-positive HLCs, which are more similar than unpurified cells to mature hepatocytes^[1].

Human

HEK293

C-6*His

P07306-1 (Q62-L291)

432  [NCBI]

Extracellular Domain

QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL

Approximately 34-48 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Derek T Peters, et al. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells. Development. 2016 May 1;143(9):1475-81.  [Content Brief]