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ASGR1/ASGPR1 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • ASGR1/ASGPR1 Protein, Human (HEK293, His) Product Image
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609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells[1].

Background

Asialoglycoprotein receptor 1 (ASGR1) has long been recognized as a hepatic surface marker and has been used to identify circulating hepatocellular carcinoma cells, purify hPSC-derived hepatocyte-like cells (HLCs) and to demonstrate the efficiency of HLC differentiation from hPSCs. ASGR1 marks a subset of albumin-positive HLCs, which are more similar than unpurified cells to mature hepatocytes[1].

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P07306-1 (Q62-L291)

  • Gene ID
    432  [NCBI]
  • Molecular Construction
    • N-term
    • ASGR1 (Q62-L291)
      Accession # P07306-1
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ASGR1; ASGR1 Protein; Asialoglycoprotein Receptor 1; ASGP-R 1; CLEC4H1; ASGPR 1; C-Type Lectin Domain Family 4 Member H1; ASGPR1; Hepatic Lectin H1; ASGPR; HL-1

  • AA Sequence

    QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL

  • Predicted Molecular Mass

    27.4 kDa

  • Molecular Weight

    Approximately 34-48 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Derek T Peters, et al. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells. Development. 2016 May 1;143(9):1475-81. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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